CD31 Rat mAb (PE-Cy7) - Primary antibody, specific to CD31

Cat. No.: Ab190235

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

390

Conjugation

PE-Cy7

Overview

Rat anti Mouse CD31 Antibody, Monoclonal (390), could be used for Flow Cytometry.

Specifications

Product Name

CD31 Rat mAb (PE-Cy7) - Primary antibody, specific to CD31

Synonyms

Cd31 | Pecam | adhesion molecule | CD31 antigen | CD31/EndoCAM | EndoCAM | FLJ34100 | FLJ58394 | GPIIA' | PECA1 | PECAM1 | PECAM-1 | PECAM-1, CD31/EndoCAM | platelet endothelial cell adhesion molecule | platelet endothelial cell adhesion molecule-1 | plat

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient traff

Host species

Rat

Specificity

CD31

Immunogen

C3H/HeJ mouse hematopoietic progenitor cell line 3

Positive Control

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugation

PE-Cy7

Excitation (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission(nm)

774nm

Ex/Em(nm)

Ex:565nm;Em:774nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD31 Rat mAb (PE-Cy7) (Ab190235)

CD31 Rat mAb (PE-Cy7) (Ab190235) - Flow Cytometry
Flow cytometry analysis of CD31 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD31 Rat mAb (PE-Cy7) (Ab190235) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy7) (Ab190073).

Flow Cytometry: CD31 Rat mAb (PE-Cy7) (Ab190235)

CD31 Rat mAb (PE-Cy7) (Ab190235) - Flow Cytometry
Flow cytometry analysis of CD31 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD31 Rat mAb (PE-Cy7) (Ab190235) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (PE-Cy7) (Ab190073). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

PECAM1 Tbio Platelet endothelial cell adhesion molecule (0 Activities)

Discover Target: PECAM1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)