Animal model of cardiac qi deficiency and blood stasis
Animal model of cardiac qi deficiency and blood stasis
The animal model of cardiac qi deficiency and blood stasis evidence reproduces the process from the main injury of blood stasis to the formation of cardiac qi deficiency evidence in normal rats, and is characterized by temporal, functional, and functionally related material changes in tissue structure. At present, the method of establishing the animal model of cardiac qi deficiency and blood stasis syndrome in Wistar rats is the coronary artery ligation method.
Principle
The basic principle of the animal model of heart qi deficiency and blood stasis is that acute myocardial infarction and blood stasis occurs when the coronary artery of rats is blocked, leading to myocardial necrosis and blood stasis for a long time and injuring the correctness of the heart, resulting in the animal model of heart qi deficiency and blood stasis.
Appliance
Animal models of heart qi deficiency and blood stasis are used to study the symptoms of heart qi deficiency and related prescription research.
Operation method
Establishment of an animal model of cardiac qi deficiency and blood stasis syndrome by coronary artery ligation method
Principle
The basic principle of coronary artery ligation method to establish the animal model of cardiac qi deficiency and blood stasis is that acute myocardial infarction and blood stasis occurs when the coronary artery of the rat is blocked, leading to myocardial necrosis and blood stasis for a long time and injuring the correctness of the heart, resulting in cardiac qi deficiency and blood stasis animal model.
Materials and Instruments
Experimental animals: Wistar rats. Move Coronary artery ligation was used to establish an animal model of cardiac qi deficiency and blood stasis as follows: A, the modeling rats were anesthetized intraperitoneally with 1% sodium pentobarbital 50 mg/kg and fixed in dorsal position. B, tracheal intubation, connected to a ventilator, and artificial respiration was performed at 80 beats/minute with a tidal volume of 0.7-0.8 ml. C, Prepare skin in the left chest between the 3rd and 4th intercostal space, disinfect, cut the skin 15 cm long, bluntly separate the muscle layer, and open the chest. D, Chest apparatus was used to expand the surgical field, and the pericardium was cut open. E, The left coronary artery was ligated approximately 1 mm below between the arterial cone and the left auricle in the modeling group of rats. F, When the electrocardiogram I lead showed significant ST-segment elevation, the heart was reset in the chest cavity and the chest wall was immediately sutured in layers. G, After the animals were observed to have stable heart rhythm and respiration, artificial respiration was stopped. H, Penicillin was injected for 3 consecutive days to prevent infection. I, The surgical procedure of rats in the sham-operated group was the same as above, but the left coronary artery was not ligated. For more product details, please visit Aladdin Scientific website.