Currently, the clinical causes of femoral head necrosis can be divided into two categories: traumatic and non-traumatic. Traumatic necrosis of the femoral head is mainly due to the sudden destruction of the blood supply of the femoral head caused by trauma, coupled with the original limited blood supply of the femoral head, resulting in ischemic necrosis. The main causes of non-traumatic necrosis of the femoral head are excessive alcohol consumption, long-term use of hormones and decompression sickness.
Principle
The basic principle of [Animal model of ischemic necrosis of the femoral head]: There are three main pathogenic mechanisms of hormonal necrosis of the femoral head: fat embolism, altered coagulation mechanism and osteoporosis. At present, most scholars believe that hormone-induced osteonecrosis of the femoral head is mainly due to ischemia of the femoral head caused by fat embolism. However, the exact pathogenesis is still unknown.
Operation method
Animal model of ischemic necrosis of the femoral head
Principle
The basic principle of [Animal model of ischemic necrosis of the femoral head]: There are three main pathogenic mechanisms of hormonal necrosis of the femoral head: fat embolism, altered coagulation mechanism and osteoporosis. At present, most scholars believe that hormone-induced osteonecrosis of the femoral head is mainly due to ischemia of the femoral head caused by fat embolism. However, the exact pathogenesis is still unknown.
Materials and Instruments
Material: Adult healthy rabbit Move The basic modeling method of the animal model of ischemic necrosis of the femoral head can be divided into the following steps: For more product details, please visit Aladdin Scientific website.
Reagents:
① Prednisolone acetate
② physiological saline
A. Adult healthy rabbits were selected, and the animals in the hormone induction group were injected with prednisolone acetate at a dose of 7.5 mg/kg twice a week for a total of 8 weeks.
B. The control group was fed under the same conditions, and was injected with physiological saline at regular intervals.
C. The femoral head was examined by taking femoral head X-ray film at regular intervals for 2, 4, 6, and 8 weeks to check for changes in the arrangement of the bone trabeculae and the articular surface, as well as for the presence of fracture, hyperplastic sclerosis, and cystic degeneration. C. X-rays of the femoral head were taken periodically at 2, 4, 6 and 8 weeks to check the trabecular arrangement of the bone and the changes of the articular surface, as well as the presence of fracture, hyperplasia, sclerosis and cystic changes in the femoral head.