The animal model of Liver-Yang hyperactivity syndrome is the main objective manifestations such as emotional irritability, capillary filling, skin flushing, accelerated heart rate, as well as subjective symptoms such as dizziness, dizziness, bitter mouth and dry throat, and insomnia, which are similar to the symptoms of patients with hypertension in the clinic. At present, there are two methods of modeling the animal model of liver-yang hyperactivity syndrome: dopamine injection method and renal artery stenosis plus infusion of epiglottis method.
Principle
The basic principle of dopamine injection is that dopamine is the precursor of adrenaline synthesized in the body, with β-receptor agonism and some a-receptor agonism, and the intravenous injection can make the animals appear similar to the manifestation of hepatic yang hyperactivity.
The basic principle of renal artery stenosis plus infusion of epiphyllum is that epiphyllum is a hot and pungent product that warms yang and helps heat, and the method of double kidney double clamping plus infusion of epiphyllum can induce hyperactivity of liver yang in animals.
Appliance
The animal model of Liver-Yang hyperactivity syndrome can be used for the study of Liver-Yang hyperactivity syndrome and related prescription research.
Operation method
Renal artery stenosis plus infusion of epiphyllum method to replicate an animal model of liver-yang hyperactivity syndrome
Principle
The basic principle of the renal artery stenosis plus infusion of epiphyllum method is that epiphyllum is a pungent, warm-yang and heat-supporting product, and the use of double-kidney double-clamping plus infusion of epiphyllum soup can induce hyperactivity of liver yang in the animal.
Materials and Instruments
Laboratory animals: Move The method of replicating the animal model of liver-yang hyperactivity syndrome by renal artery stenosis with the infusion of epimedium is as follows: Caveat 1. In the case of moulding, surgical operations should be carried out in such a way as to minimize injury and take care to prevent infection. For more product details, please visit Aladdin Scientific website.
① Rats.
Equipment:
① A set of medical surgical instruments.
Reagents:
① Sodium pentobarbital;
Penicillin.
A. The rats were anesthetized by intraperitoneal injection of pentobarbital sodium in the supine position and fixed.
B. Under aseptic conditions, the rats were dissected longitudinally along the midline of the abdomen, and the bilateral renal arteries were separated bluntly in turn, and the beginnings of bilateral renal arteries were clipped with a special silver clamp with the inner diameter of 0.20-0.23 mm.
C. The rats were injected with 40,000-80,000 U Penicillin was injected intraperitoneally to prevent infection, and then sutured layer by layer.
D. After wound healing at 2 weeks postoperatively, 20 mL/kg of epiphyllum was administered by gavage once a day for 4 consecutive weeks.