Animal model of sciatic nerve defect
Animal model of sciatic nerve defect
Peripheral nerve injury is a common limb injury, often resulting in the loss of the corresponding function of the limb. Simple cuts can be directly anastomosed, but nerve injuries with large nerve defects that are difficult to be directly anastomosed have always been difficult to treat clinically, and have also been a challenge and a hot spot in research. Currently, autologous sensory nerves are often used as reconstruction materials for nerve defect repair, but the limited source of normal nerves and the tendency to cause secondary injuries directly limit its wide application. Therefore, the search for effective materials for nerve replacement therapy has become one of the research contents of nerve defect repair.
Principle
The basic principle of [Animal model of sciatic nerve defect] is to surgically create a long segmental nerve defect in experimental animals, thus providing the necessary conditions to study the repair of peripheral nerve defects. The sciatic nerve is often used as a modeling site because it is large, easy to expose, and has typical symptoms after injury.
Operation method
Animal model of sciatic nerve defect
Principle
The basic principle of [Animal model of sciatic nerve defect] is to surgically create a long segmental nerve defect in experimental animals, thus providing the necessary conditions for the study of repair of peripheral nerve defects. The sciatic nerve is often used as a modeling site because it is large, easy to expose, and has typical symptoms after injury.
Materials and Instruments
Material: Wistar rat Move The basic method of modeling sciatic nerve defects can be divided into the following steps: . Wistar rats weighing about 250 g were anesthetized with 3% sodium pentobarbital (40 mg/kg). B. Under aseptic conditions, an oblique straight incision was made in the posterior femur to expose the sciatic nerve, and the nerve was removed about 2 em from the infraspinatus foramen at a distance of about 1 cm to create a defect. For more product details, please visit Aladdin Scientific website.
Reagent: 3% sodium pentobarbital (40 mg/kg)
A
C. After the operation, the rats were housed in separate cages.
D. Judgment criteria: Postoperative paralysis of muscles in the innervated area of the ipsilateral limb, muscle strength of grade 0, and detoriation of the triceps muscle by the triceps muscle were taken as the markers of the success of the modeling.