Cell growth curve experiment
Cell growth curve experiment
Cell growth curves can be applied to (1) determine the absolute cell growth number and (2) determine cell viability.
Operation method
Cell growth curve
Principle
The cell growth curve is a common method for determining the absolute cell growth number, which is an important indicator for determining cell viability. Generally speaking, after cell generation, cells undergo a short suspension and then wall attachment, followed by a latent period of varying lengths, i.e., they enter the exponential growth phase of massive division. In order to accurately describe the dynamic changes in cell number during the whole process, a typical growth curve can be divided into four parts: the latent phase with slow growth, the exponential growth phase with a larger slope, the flat-topped phase with a plateau-like shape, and the degradation and demise. Determination of the cell growth curve can generally be carried out using the cell counting method.
Materials and Instruments
Cells Move 1. Take cells in a good state of growth and digest them using the usual passaging methods to make a cell suspension. Caveat 1. Although the cell growth curve is the most commonly used, sometimes its reflection value is not precise enough, there can be 20-30% error, need to be analyzed in conjunction with other indicators. 2. Nowadays, many laboratories utilize culture plates to determine the growth curve using the MTT method, which is relatively simple. Common Problems 1. The standard cell growth curve is similar to an "S" shape, generally the number of cells decreases on the first day after passaging, then after a few days of latent adaptation, then enters the logarithmic growth period, reaches a plateau period after growth stabilization, and finally arrives at the senescence. 2. 2. The time to increase the number of cells by a factor of 1 on the growth curve is called the cell multiplication time, which can be converted from the curve. For more product details, please visit Aladdin Scientific website.
D-Hanks Liquid Bovine Serum RPMI1640 Dual Antibody 0.08% Trypsin
Purification bench Centrifuge Constant temperature water bath Refrigerator Inverted phase contrast microscope Incubator Straws Culture flasks Pipette tips Gun head 24 culture plates Gum plugs Micro-sampling gun Red blood cell counting plate
2. count, accurately inoculate the cells into 21-30 uniformly sized culture bottles or culture wells respectively (24-well culture plates are preferred), the total number of cells in each bottle or well is required to be the same, and the amount of culture solution added should be the same.
3. Three vials (wells) of cells were removed daily or every other day for counting and mean values were calculated. Uncounted cells were often changed for 3 to 5 d of culture.
4. Take the incubation time as the horizontal axis and the number of cells as the vertical axis (logarithmic), depicted on semi-logarithmic coordinate paper, and connect to a curve to form the growth curve of the cell.