Chicken embryo inoculation technique
Chicken embryo inoculation technique
Chicken embryo inoculation technique has the following advantages: (1) Chicken embryo is an organism with distribution of nerves and blood vessels and the structure of organs; (2) Chicken embryo has a low degree of tissue differentiation, which allows it to choose the appropriate route of inoculation, and the virus is easy to reproduce, and the infected virus contains a large number of viruses in its membranes and fluids; (3) Chicken embryo is a sufficient source, which itself seldom carries viruses and bacteria, and at the same time, it is very sensitive and does not produce antibodies to inoculated viruses. does not produce antibodies. Chicken embryo inoculation technique also has shortcomings: (1) the chicken embryo itself may carry viruses, which will affect the virus inoculation and experimental results; (2) many viruses have species specificity, and can not be inoculated with all viruses in culture; (3) in addition to the virus that causes the death of the chicken embryo and the production of confirmed scars of the virus, usually does not produce specific indications of infection, and must be utilized in addition to the experiments to prove the existence of the virus, such as hemagglutination and hemagglutination inhibition test to identify influenza viruses, the virus is not a virus, and the virus is not a virus. Hemagglutination inhibition test to identify influenza viruses; (4) chicken ingested antibiotics will remain in the body, may be passed to the chicken embryo so that rickettsiae and psittacosis and other pathogens reproduction is inhibited. These unfavorable factors affect the application of chicken embryo inoculation methods. Chicken embryo inoculation methods mainly include: amniotic cavity inoculation, chorionic urothelial membrane inoculation, urothelial cavity inoculation, and yolk sac inoculation.
Principle
The principle of the chicken embryo inoculation technique is to inoculate chicken embryos with viruses such as cowpox virus (vaccinia virus) and chicken Newcastle-disease virus (Newcastle-disease virus). The cowpox virus is suitable for growth on the chorioallantoic membrane and, when cultured, produces white pox-like lesions visible to the naked eye, resembling nodules or small white cataracts. Chicken Newcastle Disease Virus is suitable for inoculation in the allantoic cavity and amniotic cavity, after growth, the chicken embryo whole body skin hemorrhagic spots, to the back of the brain is the most significant. Chicken embryo culture method is used to culture certain animal viruses that are sensitive to chicken embryo, and this method can be used for the isolation and culture of various viruses, titration of virulence, neutralization test, and preparation of antigen and vaccine.
Appliance
The chicken embryo inoculation technique is widely used in the study of viruses, rickettsiae and chlamydiae, especially in the study of viruses, ortho/paramyxoviruses and scarab viruses.
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