Columbia Blood Agar Base (Powder)

Directions per Liter:

Dissolve 43grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Prepare 5 to 10% blood agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45-50°C. Dispense into Petri dishes or tubes.

Component	g/liter
Casein Digest Peptone	5
Corn Starch	1
Peptic Digest of Animal Tissue	8
Sodium Chloride	5
Yeast Enriched Peptone	10
Agar	14
	Total: 43g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.