Enzymatic standard curve production experiment

Summary

Master the production of enzyme standard curve, the determination of enzyme fixed time method and the use of semi-automated analyzer. This experiment is from Mudanjiang Medical College undergraduate 5-year laboratory guide for testing majors.

Operation method

Enzymatic standard curve production experiment

Principle

L-alanine + α-ketoglutarate ALT pyruvate + L-glutamate pyruvate + NADH + H+ → L-lactate + NAD+

Move

Laboratory reagents and instruments: ALT / GPT reagent standards to be measured Semi-automatic biochemical analyzer Test tubes Sampler

Second, the experimental operation:

1. Dilute the reagents.

2. Turn on the machine and preheat it to 37 degrees Celsius.

3. Select the measurement program.

4. Test:

III. Experimental results: Plot the measured data as ΔA/min-U/L and draw the standard curve.

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