Knowledge of heterochromatic granule staining; knowledge of the morphological staining characteristics of Corynebacterium diphtheriae.
Operation method
Morphological observation of diphtheria rod-shaped bacilli
Principle
Corynebacterium diphtheriae is a gram-positive bacillus with heterochromatic granules and a specific arrangement. Move Observe slices of Corynebacterium diphtheriae Gram stained and heterogeneous stained granular stained specimens. For more product details, please visit Aladdin Scientific website.
Observation of culture characteristics of Corynebacterium diphtheriae: Observation of colony characteristics of Corynebacterium diphtheriae on Lüscher serum slants and potassium tellurite blood plates.
Heterogeneous staining of particles: Neisser's staining method
Neisser staining method: Take the culture of Corynebacterium diphtheriae cultured in Lüscher's serum slant and mix the plate with physiological saline. Add the mixture of the first and second solution of Neisser's stain to the flame-fixed specimen, stain for 1-3 minutes, wash with water, and then stain with the third solution for half a minute, pour off the stain, and then dry for microscopic examination. The body of Corynebacterium diphtheriae was yellow-brown, and the heterochromatic particles were blue-black.