Acute pancreatitis (acute pancreatitis) is one of the common acute abdominal diseases in surgery, with increasing incidence in recent years and a wide range of hazards. Among them, severe acute pancreatitis has attracted great attention from scholars because of its dangerous condition and high mortality rate. At present, there are many ways to prepare animalmodels of acute pancreatitis, which are mainly divided into invasive and non-invasive types. At present, there is one main method for modeling animalmodels of acute pancreatitis: L-arginine intraperitoneal injection method for preparing pancreatitis models.
Principle
The basic principle of modeling experiments in animal models of acute pancreatitis is that common etiologies of pancreatitis include obstruction, excessive alcohol consumption, overeating, hypercalcemia, trauma, and pancreatic ischemia, etc. The above etiologies may lead to excessive pancreatic hypersecretion, impaired pancreatic fluid excretion, circulatory disturbances in the pancreatic blood system, and reduction of physiologic pancreatic protease inhibitors, which can activate the pancreatic enzymes and digest the pancreas itself through a common mechanism, thereby triggering Acute pancreatitis.
Operation method
Preparation of pancreatitis model by intraperitoneal injection of L-arginine
Principle
The basic principle of L-arginine intraperitoneal injection for the preparation of acute pancreatitis model in rats is that the injection of L-arginine activates pancreatic enzymes and digests the pancreas itself and the surrounding tissues leading to the development of pancreatitis.
Materials and Instruments
Equipment: Move The basic procedure of L-arginine intraperitoneal injection for the preparation of pancreatitis model can be divided into the following steps: A Firstly, 0.9 g of arginine was weighed and mixed with 15 mL of physiological saline to form a reagent with a concentration of 6%. B The rats were anesthetized with ether inhalation, and after successful anesthesia, the rats were fixed in the surgical animal experiment table. C Peritoneal puncture was performed 1 cm to the right of the midline of the rat's abdomen and 7.5 mL of prepared arginine injection was injected; after the injection, the rat was observed for 1 hour, and then another peritoneal puncture was performed 1 hour later and 7.5 mL of arginine injection was injected. For more product details, please visit Aladdin Scientific website.
Surgical animal bench, syringes, feeding cages, healthy rats, etc.
Reagents:
① Arginine.
②Ether