Immunoglobulins are the central effector molecules of adaptive humoral immunity. They are produced by B cells and their terminally differentiated plasma cells and contribute to pathogen clearance, maintenance of immune homeostasis, and the development of immunopathology through a dual ...
Topic
Monoclonal antibody
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
4 resources · protocols, FAQs & articles
Hybridoma technology, as the classical method for monoclonal antibody (mAb) production, is widely applied in immunology research, diagnostic reagent development, and antibody drug manufacturing. Because hybridom a cells are highly sensitive to culture conditions and reagent quality, the reagents ...
Immunohistochemistry staining with samples in paraffin (IHC-P)
IgG, the key factor of Humoral immunity response, is the main immunoglobulin in blood, lymph, cerebrospinal fluid and peritoneal fluid. IgG accounts for approximately 75% of total serum immunoglobulins and is the only immunoglobulin that can penetrate the human placenta. It is primarily ...
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Techniques often explored alongside immunological experiments.