G-749 - Moligand™, ≥98% , Inhibitor of fms related receptor tyrosine kinase 3;Inhibitor of MER proto-oncogene; tyrosine kinase, CAS No.1457983-28-6, Inhibitor of fms related receptor tyrosine kinase 3;Inhibitor of MER proto-oncogene; tyrosine kinase

CAS: 1457983-28-6 Cat. No.: G413776 Formula: C₂₅H₂₅BrN₆O₂ Molecular Weight: 521.41

GRADE & PURITY Moligand™, ≥98%

Synonyms

38-bromo-11-methyl-6-oxa-2,4-diaza-3(2,4)-pyrido[4,3-d]pyrimidina-1(4)-piperidina-5(1,4),7(1)-dibenzenaheptaphan-35(36H)-one | UNII-B06W426B66 | A899265 | Q7825530 | 1457983-28-6 | Q27077763 | G 749 | MFCD28167815 | 8-Bromo-2-[(1-methyl-4-piperidinyl)amin

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Information

G-749 G-749 is a novel and potent FLT3 inhibitor with IC50 of 0.4 nM, 0.6 nM and 1 nM for FLT3 (WT), FLT3 (D835Y), and Mer, respectively, showing lower potency against other tyrosine kinases.

Targets

FLT3 (Cell-free assay); FLT3 (D835Y) (Cell-free assay); Mer (Cell-free assay); Aurora B (Cell-free assay); RET (Cell-free assay) 15501,0.4 nM; 0.6 nM; 1 nM; 6 nM; 9 nM

In vitro

In FLT3-WT-bearing RS4-11 cells and FLT3-ITD-harboring MV4-11 and Molm-14 cells, G-749 potently inhibits autophosphorylation of FLT3 with IC50 of ≤8 nM. In leukemia cells, G-749 shows antiproliferative activity by inducing apoptosis. In BaF3 cell lines that stably express FLT3-ITD/N676D, FLT3-ITD/F691L, FLT3-D835Y, or FLT3-D835Y/N676D, G-749 shows strong inhibitory activity with IC50 of <10 nM, and thus overcomes drug resistance. In blasts of AML patients, G-749 also exhibits potent antileukemia activity.

In vivo

In MV4-11 xenograft mice, G-749 (30 mg/kg p.o.) effectively inhibits the FLT3 pathway and significant inhibits tumor growth. In an orthogonal model of bone marrow engraftment using Molm-14 cells, G-749 (20 mg/kg p.o.) also inhibits tumor growth and increases survival.

Cell Research(from reference)

Cell lines：MV4-11, RS4-11, K562, HEL, and Molm-14 cells 

Concentrations：~1 μM 

Incubation Time：72 hours 

Specifications

Synonyms

38-bromo-11-methyl-6-oxa-2,4-diaza-3(2,4)-pyrido[4,3-d]pyrimidina-1(4)-piperidina-5(1,4),7(1)-dibenzenaheptaphan-35(36H)-one | UNII-B06W426B66 | A899265 | Q7825530 | 1457983-28-6 | Q27077763 | G 749 | MFCD28167815 | 8-Bromo-2-[(1-methyl-4-piperidinyl)amin

Specifications & Purity

Moligand™, ≥98%

Biochemical and Physiological Mechanisms

G-749 is a novel and potent FLT3 inhibitor with IC50 of 0.4 nM, 0.6 nM and 1 nM for FLT3 (WT), FLT3 (D835Y), and Mer, respectively, showing lower potency against other tyrosine kinases.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Action Type

INHIBITOR

Mechanism of action

Inhibitor of fms related receptor tyrosine kinase 3;Inhibitor of MER proto-oncogene; tyrosine kinase

Purity

≥98%

Names and Identifiers

• Pubchem Sid: 504772431
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504772431
• Canonical Smiles: CN1CCC(CC1)NC2=NC3=C(C(=O)NC=C3Br)C(=N2)NC4=CC=C(C=C4)OC5=CC=CC=C5
• IUPAC Name: 8-bromo-2-[(1-methylpiperidin-4-yl)amino]-4-(4-phenoxyanilino)-6H-pyrido[4,3-d]pyrimidin-5-one
• InChIKey: SXWMIXPJPNCXQQ-UHFFFAOYSA-N
• INCHI: 1S/C25H25BrN6O2/c1-32-13-11-17(12-14-32)29-25-30-22-20(26)15-27-24(33)21(22)23(31-25)28-16-7-9-19(10-8-16)34-18-5-3-2-4-6-18/h2-10,15,17H,11-14H2,1H3,(H,27,33)(H2,28,29,30,31)
• Isomeric SMILES: CN1CCC(CC1)NC2=NC3=C(C(=O)NC=C3Br)C(=N2)NC4=CC=C(C=C4)OC5=CC=CC=C5
• Alternate CAS: 1457983-33-3
• Molecular Weight: 521.41
• Reaxy-Rn: 24014667
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=24014667&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Benzenoids
    • Class Benzene and substituted derivatives
      • Subclass Diphenylethers

• Kingdom: Organic compounds
• Superclass: Benzenoids
• Class: Benzene and substituted derivatives
• Subclass: Diphenylethers
• Intermediate Tree Nodes: Not available
• Direct Parent: Diphenylethers
• Alternative Parents: Diarylethers Pyridopyrimidines Aniline and substituted anilines Phenoxy compounds Phenol ethers Aminopyrimidines and derivatives Pyridinones Aryl bromides Piperidines Imidolactams Vinylogous amides Heteroaromatic compounds Trialkylamines Lactams Azacyclic compounds Hydrocarbon derivatives Organic oxides Organobromides
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Diphenylether - Diaryl ether - Pyridopyrimidine - Phenol ether - Aniline or substituted anilines - Phenoxy compound - Aminopyrimidine - Pyridinone - Pyridine - Piperidine - Imidolactam - Pyrimidine - Aryl bromide - Aryl halide - Heteroaromatic compound - Vinylogous amide - Lactam - Tertiary aliphatic amine - Tertiary amine - Ether - Azacycle - Organoheterocyclic compound - Organobromide - Organohalogen compound - Organic nitrogen compound - Amine - Hydrocarbon derivative - Organic oxide - Organic oxygen compound - Organonitrogen compound - Organooxygen compound - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as diphenylethers. These are aromatic compounds containing two benzene rings linked to each other through an ether group.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Associated Targets(Human)

FLT3 Tclin Receptor-type tyrosine-protein kinase FLT3 (1 Activities)

Discover Target: FLT3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MERTK Tchem Tyrosine-protein kinase Mer (1 Activities)

Discover Target: MERTK

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

FLT3 Tclin Tyrosine-protein kinase receptor FLT3 (13481 Activities)

Discover Target: FLT3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

HepG2 (196354 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MV4-11 (7307 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MOLM-14 (399 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Associated Targets(non-human)

BaF3 (4657 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

rep Replicase polyprotein 1ab (378 Activities)

Discover Target: rep

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Chemical and Physical Properties

• Solubility: Solubility (25°C) In vitro DMSO: 24 mg/mL warmed with 50ºC Water: bath (46.02 mM); Water: Insoluble; Ethanol: Insoluble
• Molecular Weight: 521.400 g/mol
• XLogP3: 4.900
• Hydrogen Bond Donor Count: 3
• Hydrogen Bond Acceptor Count: 7
• Rotatable Bond Count: 6
• Exact Mass: 520.122 Da
• Monoisotopic Mass: 520.122 Da
• Topological Polar Surface Area: 91.400 Ų
• Heavy Atom Count: 34
• Formal Charge: 0
• Complexity: 716.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

Application Protocols

No tested applications or protocols are provided in the listing for this SKU.

• Recommended assay types, concentrations, and controls: Not specified for this item; refer to CoA/Spec Sheet.
• Positive control, vehicle control, or reference standards: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (for small-molecule ligands):

• Prepare fresh working solutions from frozen aliquots to minimize degradation and adsorption losses. Keep final DMSO consistent across conditions.
• Include solubility/precipitation checks in your protocol and confirm concentration by UV or LC–MS where possible.
• Capture metadata (solvent lot, buffer composition, temperature, plate type) for reproducibility.

For research use only.

Biological Roles

The product is designated for research use as a small-molecule ligand (Moligand™). The listing provides no target, pathway, or mode-of-action details for this SKU.

General notes for small-molecule ligands (no therapeutic claims):

• Such compounds are used to interrogate protein function, modulate enzymatic activity, or perturb signaling pathways in biochemical or cell-based models. They can support target validation, phenotypic screening, and mechanism-of-action studies.
• Typical experimental flow includes potency determination (e.g., IC50/EC50), selectivity profiling across related targets, and orthogonal validation (e.g., CETSA/thermal shift, chemoproteomics, or genetic knockdown/knockout comparison).
• Physicochemical properties (solubility, stability, permeability) and liabilities (aggregation, redox activity, PAINS motifs) can influence observed biological effects. In the absence of structure data, users should implement assay controls that detect such artifacts.

Item-specific biological role or target: Not specified for this item; refer to CoA/Spec Sheet and any associated application notes. Any statements of specificity or selectivity should be substantiated by your in-house data or peer-reviewed literature tied unambiguously to the CAS/CID and batch identity.

Buffer Applications

Not typically applicable. This SKU is a small-molecule ligand and not a buffering agent. It does not constitute a defined buffer system, and no pH range or buffering capacity is provided.

Practical notes for use in buffered assays (general):

• Prepare primary stocks in an appropriate organic solvent (commonly DMSO), then dilute into assay buffer while keeping the final organic co-solvent fraction low and consistent across controls and treated samples.
• Include carrier protein or mild nonionic detergent if precipitation or nonspecific adsorption is observed.
• Verify compound stability in the intended buffer (e.g., by LC–MS after incubation at assay temperature) before running critical experiments.

Item-specific buffer guidance: Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

This SKU is a discrete small-molecule screening ligand, not a process solvent or commodity reagent. Therefore, the concept of “green alternatives” (e.g., swapping to a greener solvent) is not typically applicable.

Nevertheless, greener handling practices can still apply:

• Optimize stock concentration to minimize solvent volumes (especially DMSO and DMF), and batch aliquot to reduce waste from freeze–thaw cycles.
• Use microscale assay formats and automation to lower overall chemical usage.
• Implement solvent recycling where compatible with quality requirements (e.g., recovering high-purity DMSO for non-critical cleaning steps), following institutional policies.
• Prefer aqueous assay buffers with minimal organic co-solvent content consistent with assay performance.

If you are seeking greener substitutes for solvents used with this compound:

• Consider replacing DMF with DMSO or aqueous co-solvent systems when compatible with solubility and assay integrity.
• For chromatographic purification or analytics, choose lower-toxicity mobile phase modifiers and minimize acetonitrile usage through method optimization.

Item-specific greener alternatives: Not applicable; no alternative compound delivering the same biological profile is defined in the product data.

Pharmaceutical Uses

No pharmaceutical excipient role or pharmacopeial status is provided for this SKU. The product is labeled for research use only and is not intended for clinical or diagnostic applications.

General context (for discovery-stage compounds only):

• Compounds like this may be used in pre-formulation research (e.g., solubility screening in various vehicles, stability studies) to understand developability, but such work remains strictly non-clinical unless supported by additional quality, regulatory, and safety data.
• Any consideration of GMP manufacturing, ICH stability, or formal CMC characterization lies outside the scope of this listing and requires separate qualification.

Item-specific excipient uses or regulatory listings: Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Item-specific physical constants are not included in the provided listing.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point (MP): Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point (BP): Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.
• LogP/logD: Not specified for this item; refer to CoA/Spec Sheet.
• pKa: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not applicable for typical solid screening compounds; otherwise not specified for this item.

Practical guidance (general, literature/common practice for screening compounds):

• Many small-molecule screening ligands are supplied as solids and are commonly reconstituted in anhydrous DMSO to make stock solutions (e.g., 10–50 mM), then diluted into assay buffer immediately prior to use. Confirm this approach against the CoA/SDS for this specific item before proceeding.
• Hygroscopicity and polymorphism can influence handling and apparent solubility. Inspect the CoA for any notes on hydrate/solvate content and recommended solvents.
• If the compound is an amorphous solid or low-melting solid, it may exhibit a broad or depressed MP; rely on batch-specific data.

Quality & Grades

Grade/Purity: Moligand™ (as provided).

What this typically implies:

• Moligand™ designates a small-molecule ligand suitable for screening, target validation, or chemical biology workflows. Such products are commonly accompanied by analytical quality control (e.g., LC–MS, purity by HPLC/UPLC, sometimes NMR) verifying identity and batch quality relevant to discovery research.
• UV cutoff, specific impurity profiles (e.g., water, peroxides, metals), and residual solvent content are NOT specified for this item; refer to the CoA/Spec Sheet for batch-specific details.

How to interpret grade for use:

• Screening-grade ligands are intended for in vitro and ex vivo research. If you plan to use the compound in sensitive biophysical assays (SPR, ITC, DSF) or analytical quantitation, confirm purity, counter-ion/solvate state, and residual solvent levels in the CoA.
• If orthogonal confirmation is required, consider running your own analytics (e.g., independent LC–MS, quantitative NMR) on receipt to verify identity and concentration in your assay matrix.

Batch-to-batch considerations:

• Storage and shipping conditions (frozen, cold chain) suggest stability is enhanced at low temperature; always equilibrate sealed containers to room temperature before opening to prevent moisture condensation.
• Any stabilizers or form-specific notes: Not specified for this item; refer to CoA/Spec Sheet.

Reaction & Applications

This product is positioned as a Moligand™ screening compound rather than a general-purpose synthetic reagent. Consequently, classical synthetic reaction applications (e.g., as a solvent, catalyst, or coupling partner) are not typically applicable.

Research applications (general for screening ligands):

• Chemical biology: Probe-based studies to explore target engagement in biochemical assays (enzyme inhibition/activation, receptor binding) or in cell-based readouts. Specific targets for this SKU are not provided; design assays based on your hypothesis and verify with orthogonal methods.
• Assay development: Positive/negative control benchmarking, hit confirmation, and SAR hypothesis generation in early discovery campaigns.
• Biophysical profiling: Thermal shift (DSF), microscale thermophoresis (MST), SPR/ITC—subject to compound solubility and spectral properties.

Practical considerations:

• Purity and identity confirmation via LC–MS and, when feasible, 1H NMR prior to screening reduce false positives/negatives from impurities.
• Aggregate-based inhibition can confound readouts; consider adding nonionic detergents (e.g., 0.01–0.1% Triton X-100 or Tween-20) and run detergent-sensitivity controls.
• Fluorescent/absorbing interferences: If the compound has strong chromophores (unknown for this item), assess assay signal interference using no-enzyme and no-substrate controls.

Item-specific application notes: Not provided in the listing; refer to CoA/Spec Sheet and internal validation data if available.

Reaction Conditions

Classical synthetic reaction conditions are not generally applicable because this product is supplied as a ready-made small-molecule ligand rather than a reagent or substrate for standard transformations.

If you plan to perform chemical transformations on this molecule (general advice):

• Obtain the definitive structure and purity from the CoA and verify analytically.
• Assess stability across pH, temperature, and redox conditions before attempting derivatization; many bioactive scaffolds contain sensitive heterocycles or electrophiles.
• Use small-scale test reactions to probe compatibility with acids/bases, oxidants/reductants, and transition-metal catalysts. Monitor by LC–MS.
• Protect labile motifs (if present) prior to harsh conditions; choose anhydrous, oxygen-free conditions when working with oxidation-prone motifs.

Item-specific reaction media, temperature ranges, catalysts, and yields: Not specified for this item; refer to CoA/Spec Sheet.

Safety & Handling

Hazard information is not included in the supplied data for this SKU. Always consult the SDS supplied with the product for authoritative safety and first-aid measures.

• GHS classification: Not specified for this item; refer to SDS.
• Signal word / H-statements / Pictograms: Not specified for this item; refer to SDS.

General laboratory precautions (good practice):

• Personal protective equipment: lab coat, safety glasses, and suitable chemical-resistant gloves. Handle in a chemical fume hood to avoid inhalation of dust or aerosols.
• Avoid contact with skin and eyes. Prevent ingestion and inhalation. Wash thoroughly after handling.
• Incompatibilities: Unknown for this specific item; in the absence of data, avoid strong oxidizers, strong acids/bases, and reactive reducing agents. Do not heat or aerosolize unknown solids.
• First aid (overview; defer to SDS for details):
  • Inhalation: Move to fresh air; seek medical attention if symptoms persist.
  • Skin contact: Wash with soap and water; remove contaminated clothing.
  • Eye contact: Rinse cautiously with water for several minutes; remove contact lenses if present and easy to do; seek medical advice if irritation persists.
  • Ingestion: Rinse mouth; seek medical advice. Do not induce vomiting unless directed by medical personnel.
• Spill response: Avoid dust generation; collect with inert absorbent; dispose per institutional and local regulations.
• Fire: Use CO2, dry chemical, or foam as appropriate. Combustion products are unknown; firefighting should be performed with SCBA.

Note: For research use only; not for human or veterinary use.

Solvent Selection

No item-specific solubility data are supplied. Selection should follow general best practices for small-molecule screening compounds while verifying against the CoA/SDS.

General guidance (common for discovery ligands):

• Primary stock solvent: Anhydrous DMSO is commonly used due to broad solvating power and compatibility with biochemical assays. Prepare concentrated stocks (e.g., 10–50 mM) if solubility permits, then dilute into assay buffers to ≤0.1–1% DMSO v/v final (as your biology allows).
• Alternative solvents: DMF or ethanol can be used for pre-dissolution when DMSO is unsuitable, but verify assay tolerance and compound stability.
• Aqueous compatibility: Many hydrophobic ligands precipitate on direct addition to water/buffer. Pre-dilute DMSO stock with a small volume of buffer containing carrier protein (e.g., BSA) or a co-solvent, then stepwise dilute to minimize precipitation. Confirm visually and by LC–MS/UV.

Small comparison (general):

• DMSO: highest success rate for initial dissolution; broadly assay-compatible at low %.
• DMF: similar solvency; sometimes higher cytotoxicity and assay interference than DMSO.
• Ethanol: volatile, lower solvency for very hydrophobic chemotypes; can affect enzyme activities at modest %.

Practical tips:

• Warm gently (e.g., 25–37°C) and vortex/sonicate to assist dissolution; avoid prolonged heating.
• Filter sterilization (0.22 μm PTFE) only after confirming no adsorption or degradation.
• Record exact solvent lot and water content for reproducibility.

Item-specific solubility and stability: Not specified for this item; refer to CoA/Spec Sheet.

Storage & Reconstitution

From product data:

• Storage conditions: Store at −20°C.
• Shipped in: Ice chest + ice pads (cold chain maintained during transit).

Additional handling guidance (general best practice for screening compounds; verify against CoA/SDS for this specific item):

• Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.
• After initial opening, promptly reseal under dry conditions. If feasible, aliquot into inert, airtight vials to minimize freeze–thaw cycles and headspace moisture.
• Protect from light if the compound is known or suspected to be photosensitive (item-specific photosensitivity not specified).
• Reconstitution: Not specified for this item; refer to CoA/Spec Sheet. Many users prepare DMSO stocks for small-molecule ligands; confirm suitability for this item before use.
• Stability after reconstitution: Not specified for this item; verify by analytical checks (e.g., LC–MS) at relevant timepoints.

Note: For research use only.

Structure & Identity

Brief overview: G-749 (SKU G413776) is provided as a Moligand™ screening compound. The supplier listing does not include a structure or elemental composition for this specific catalog entry.

• CAS: 1457983-28-6 (as provided)
• CID: 78357765 (as provided)
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet. (Note: the abbreviated code “348270” in the listing is not a standard InChIKey format.)
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general notes):

• Item-specific structural motifs (rings, heteroatoms, stereocenters) are not disclosed in the product data. For definitive structural details, consult the CoA/Spec Sheet or the corresponding SDS.
• If using public registry records (e.g., the stated CID), verify consistency with the batch-specific CoA before experimental use, as screening compounds can have multiple salt/solvate forms or catalog-specific identifiers.

2D structure description (general):

• Not available from the supplied data. No structural diagram or line notation has been provided for this SKU. Users should rely on the official documentation packaged with the product for exact identity confirmation (e.g., NMR/LC–MS traces referenced in the CoA).

Synthetic Utility

Not a general synthetic building block. This SKU is offered as a discrete screening ligand rather than a reagent for bond-forming chemistry.

Guidance if derivatization is contemplated (general):

• Without a disclosed structure, functional-handle availability (e.g., amine, carboxyl, halide) is unknown. Obtain the exact structure and confirm with analytical data (NMR, HRMS) before planning semisynthesis or bioconjugation.
• If analog preparation is desired for SAR, route scouting should begin from the known scaffold (once confirmed) rather than attempting late-stage diversification of the final ligand, which may be less efficient and could compromise purity.
• Protecting-group strategies, cross-coupling handles, or late-stage functionalization tactics must be guided by the verified structure and stability profile; none are specified for this item.

Item-specific synthetic roles, named-reaction participation, or retrons: Not specified for this item; refer to CoA/Spec Sheet.

Target Specificity

No target, selectivity profile, or mechanism-of-action information is provided in the product data for this SKU.

• Molecular target(s): Not specified for this item; refer to CoA/Spec Sheet.
• Potency metrics (IC50/Ki/Kd/EC50): Not specified for this item; refer to CoA/Spec Sheet.
• Off-target panel, kinome profiling, receptor selectivity: Not specified for this item; refer to CoA/Spec Sheet.

Notes:

• If target engagement is part of your use case, perform orthogonal confirmation (e.g., enzymology vs. biophysical binding) and include counterscreens against related proteins to establish selectivity.
• Ensure all claims are tied to the exact CAS/CID and batch identity to avoid misattribution.