Light Green SF Yellowish - AR , CAS No.5141-20-8

CAS: 5141-20-8 Cat. No.: L113832 Molecular Weight: 792.85 Beilstein Registry Number: 5717828 EC Number: 225-906-5
AVAILABLE TO ORDER
GRADE & PURITY AR ? Analytical Reagent grade — high-purity chemicals meeting strict assay limits for lab analysis. Use when accuracy matters and trace impurities could skew results.
Synonyms
D&C Green No. 4 | A.F. Green No. 2 | Light SF Yellowish (Biological stain) | C.I. ACID GREEN 5, DISODIUM SALT | FD & C green no. 2 | Light Green 2GN | Light Green SF Yellowish | sodium 3-(((E)-ethyl((E)-4-((4-(ethyl(3-sulfonatobenzyl)amino)phenyl)(4-sulfo
Storage
Room temperature
Shipped In
Normal
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
1g
L113832-1g
10
$10.90
5g
L113832-5g
4
$39.90
25g
L113832-25g
4
$141.90
100g
L113832-100g
2
$509.90
Enter a quantity for the sizes you want to add.
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Why this grade

AR AR for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Room temperature Ships Normal Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 3 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Light green SF yellowish (LGSFY) is a disodium salt of an acid triphenylmethane dye. It is also known as Light Green, Lissamine Green SF, Food Green 2, Green No. 205, Acid Green, Acid Brilliant Green 5, and Acid Brilliant Green SF. LGSFY is an important component of Papanicolaou stains in addition to eosin Y and bismark brown Y. Light green SF yellowish is a histological stain for collagen and a permitted colorant for foodstuffs and beverages. Product Application
Light green SF is generally used as a cytoplasmic counterstain with nuclear stains like safranine and Feulgen stain. In histopatology, it is used for collagen fiber staining in Masson′s trichrome, Papanicolaou cytological polychrome stains, and Twort stain for microorganisms in tissue sections. It is a constituent of polychrome staining method used for differentiating secretory cells of the pituitary gland. Light green SF also stains the cellulose cell walls of plant cells.

Specifications

Synonyms
D&C Green No. 4 | A.F. Green No. 2 | Light SF Yellowish (Biological stain) | C.I. ACID GREEN 5, DISODIUM SALT | FD & C green no. 2 | Light Green 2GN | Light Green SF Yellowish | sodium 3-(((E)-ethyl((E)-4-((4-(ethyl(3-sulfonatobenzyl)amino)phenyl)(4-sulfo
Specifications & Purity
AR
Biochemical and Physiological Mechanisms
Triarylmethane dye. Cytological counterstain that can be used as a substitute for aniline blue in Gomori′s one-step trichrome procedure. An important contrast stain component of Papanicolaou (PAP) stains.
Storage
Room temperature
Shipped In
Normal
Grade
AR
Note
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Names and Identifiers
Pubchem Sid488182677
Pubchem Sid Urlhttps://pubchem.ncbi.nlm.nih.gov/substance/488182677
Canonical SmilesCCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3)C5=CC=C(C=C5)S(=O)(=O)[O-].[Na+].[Na+]
IUPAC Namedisodium;3-[[N-ethyl-4-[[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]cyclohexa-2,5-dien-1-ylidene]-(4-sulfonatophenyl)methyl]anilino]methyl]benzenesulfonate
InChIKeyDGOBMKYRQHEFGQ-UHFFFAOYSA-L
INCHI1S/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2
Isomeric SMILES CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3)C5=CC=C(C=C5)S(=O)(=O)[O-].[Na+].[Na+]
WGK Germany 2
RTECS BQ4900000
Molecular Weight 792.85
Beilstein 5717828
Reaxy-Rn 5717828
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=5717828&ln=

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

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✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

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📊 Datasheet

Quick-reference summary of product specifications and applications.

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🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

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Advanced Data

3D Structure
Interactive Chemical Structure Model





Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

29 results found

Lot NumberCertificate TypeDateItem
J2520634Certificate of AnalysisSep 02, 2025 L113832
J2520633Certificate of AnalysisSep 02, 2025 L113832
J2520632Certificate of AnalysisSep 02, 2025 L113832
J2520578Certificate of AnalysisSep 02, 2025 L113832
E2607039Certificate of AnalysisSep 02, 2025 L113832
D2113107Certificate of AnalysisJan 09, 2025 L113832
H2518042Certificate of AnalysisJul 12, 2024 L113832
G2431284Certificate of AnalysisJul 12, 2024 L113832
G2431283Certificate of AnalysisJul 12, 2024 L113832
G2431282Certificate of AnalysisJul 12, 2024 L113832
G2003117Certificate of AnalysisApr 07, 2024 L113832
E23091002Certificate of AnalysisFeb 03, 2023 L113832
E2309991Certificate of AnalysisFeb 03, 2023 L113832
E2309989Certificate of AnalysisFeb 03, 2023 L113832
E2309985Certificate of AnalysisFeb 03, 2023 L113832
E2309979Certificate of AnalysisFeb 03, 2023 L113832
E2309640Certificate of AnalysisFeb 03, 2023 L113832
E2309492Certificate of AnalysisFeb 03, 2023 L113832
E23091006Certificate of AnalysisFeb 03, 2023 L113832
L2205731Certificate of AnalysisOct 09, 2022 L113832
L2205729Certificate of AnalysisOct 09, 2022 L113832
L2205636Certificate of AnalysisOct 09, 2022 L113832
L2205735Certificate of AnalysisOct 09, 2022 L113832
L2205635Certificate of AnalysisOct 09, 2022 L113832
L2205634Certificate of AnalysisOct 09, 2022 L113832
I2222271Certificate of AnalysisAug 22, 2022 L113832
I2222270Certificate of AnalysisAug 22, 2022 L113832
I2222263Certificate of AnalysisAug 22, 2022 L113832
H2131374Certificate of AnalysisAug 10, 2021 L113832

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Chemical and Physical Properties
SolubilitySoluble in water
Melt Point(°C)288°C
Molecular Weight792.900 g/mol
XLogP3
Hydrogen Bond Donor Count0
Hydrogen Bond Acceptor Count10
Rotatable Bond Count9
Exact Mass792.122 Da
Monoisotopic Mass792.122 Da
Topological Polar Surface Area203.000 Ų
Heavy Atom Count53
Formal Charge0
Complexity1500.000
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
The total count of all stereochemical bonds0
Covalently-Bonded Unit Count3
Documents & Articles
Citations of This Product
References
1. Shirong Kang, Hao Zhang, Mingyu Dou, Gaimei Gao, Hongjie Zhu, Hua Yang, Dacheng Li, Jianmin Dou.  (2022)  The microthermal construction of Z-scheme CdS@g-C3N4 composite: Efficient tetracycline photodegradation, reaction mechanism and possible degradation pathway.  OPTICAL MATERIALS,      [PMID:] [10.1016/j.optmat.2022.112092]
2. Congcong Pei, Aaron Albert Aryee, Keke Zhu, Rong Wang, Runping Han.  (2024)  Adsorption and catalytic degradation of Light green SF by magnetic PEI-modified carbon nanotubes composites.  SEPARATION AND PURIFICATION TECHNOLOGY,      [PMID:] [10.1016/j.seppur.2024.129625]
3. Qingwen Tang, Lichao Xia, Lie Liu, Xiaoqiang An, Huachun Lan, Huijuan Liu, Jiuhui Qu.  (2024)  Polymeric loose nanofiltration membranes based on water-soluble carbon nitride for efficient organics/salts separation.  JOURNAL OF MEMBRANE SCIENCE,      [PMID:] [10.1016/j.memsci.2024.123687]
Solution Calculators
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