Pyruvate Kinase (PK), CAS No.9001-59-6

CAS: 9001-59-6 Cat. No.: P1510309

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Rabbit muscle; ≥200 U/mg enzyme powder

Accession #

P11974

Bioactivity

≥200 U/mg enzyme powder

Overview

Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L, R, M1, and M2. The L isozyme is localized in gluconeogenic tissues, particularly in the liver. Whereas the R and M1 are localized in the adult skeletal muscles, heart, brain and erythrocytes, respectively. M2 is localized in the nucleus of the cells.

Reaction

ATP + pyruvate = ADP + phosphoenolpyruvate

Action

Pyruvate kinase catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP.

This transfer yields one molecule of pyruvate and one molecule of ATP.

Application

Enzymatic determination of ADP, ATP, pyruvate or phsphoenolpyruvate (PEP) when coupled with the related enzymes.

Specifications

Product Name

Pyruvate Kinase (PK), CAS No.9001-59-6

Synonyms

ATP:pyruvate 2-O-phosphotransferase | pyruvate kinase | phosphoenolpyruvate kinase | phosphoenol transphosphorylase | PKM | PKM2

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Rabbit muscle; ≥200 U/mg enzyme powder

Biochemical and Physiological Mechanisms

Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whe

Bioactivity

≥200 U/mg enzyme powder

Accession #

P11974

Predicted molecular weight

237 kDa (as a tetramer of four equal subunits 57 kDa)

CAS

9001-59-6

Enzyme Commission Number

EC 2.7.1.40

Molecule Type

Enzyme

Storage and Shipping

Concentration

from Rabbit muscle; ≥200 U/mg enzyme powder

Reconstitution

Reconstitute in 0.9% saline. Prepare further dilutions in tris buffered saline with 1% BSA. Aliquot and freeze at -20°C after reconstitution.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (36 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

One unit will catalyze the conversion of 1.0 micromole of phosphoenolpyruvate to pyruvate per minute at pH 7.6 at 37°C.