Pyruvic Acid (PA) Content Assay Kit (LDH, Micro Method)

Cat. No.: P1515881

GRADE & PURITY BioReagent

Overview

Pyruvic acid (PA), also known as 2-oxopropanoic acid, is a pivotal intermediate in fundamental metabolism across organisms. It serves as a critical metabolic hub, enabling the interconversion of carbohydrates, fats, and amino acids via acetyl-CoA and the tricarboxylic acid (TCA) cycle. Both pyruvate and lactate are products of anaerobic glycolysis. Researchers often study them together, using their ratio to estimate the degree of circulatory failure. Methods for pyruvate detection include the lactate dehydrogenase (LDH) catalytic assay and the dinitrophenylhydrazine method. The dinitrophenylhydrazine method, while straightforward due to visible color formation, is less specific (susceptible to interference from α-keto acids), more cumbersome, and is now considered outdated. The preferred current method is the lactate dehydrogenase catalytic assay. The detection principle of this kit is based on the LDH-catalyzed oxidation of pyruvate to lactate in the presence of NADH, generating NAD⁺. Under weakly alkaline conditions, the reaction equilibrium favors the oxidation of pyruvate to lactate. By measuring the rate of decrease in NADH absorbance at 340 nm using a microplate reader, the pyruvate concentration can be calculated. This kit is suitable for detecting endogenous pyruvate levels in samples such as cell or tissue lysates/homogenates, plasma, and serum. This product is intended for research use only and is not suitable for clinical diagnosis or other purposes.

Storage and Shipping

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ for 6 months. Store in the dark.