GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human ARID1A Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant ARID1A Antibody - Primary antibody, specific to ARID1A, Rabbit IgG
Synonyms
actin-dependent regulator of chromatin subfamily F member 1 antibody | ARI1A_HUMAN antibody | ARID domain containing protein 1A antibody | ARID domain-containing protein 1A antibody | ARID1A antibody | AT rich interactive domain 1A (SWI like) antibody | A
Specifications & Purity
Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically. Also involved in vitamin D-coupled transcription regulation via its association with the WIN
Host species
Rabbit
Specificity
ARID1A
Immunogen
A synthetic peptide derived from human ARID1A (AA 1200-1400).
Positive Control
WB: HEK293, Jurkat and HeLa cell lysates. IHC: Human colon adenocarcinoma tissue. IF/ICC: SH-SY5Y cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant ARID1A Antibody (Ab089579) - Western Blot All lanes: Recombinant ARID1A Antibody (Ab089579) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 242 kDa Observed band size: 132, 150, 270 kDa ARID1A has many mutations which typically generate truncated proteins that are highly prone to degradation. (PMID: 21614196, PMID: 29486633, PMID: 34429326). Exposure time: 17.3 s
Recombinant ARID1A Antibody (Ab089579) - Western Blot All lanes use Recombinant ARID1A Antibody (Ab089579) at 1/1000 dilution for 1 hour at room temperature.
Recombinant ARID1A Antibody (Ab089579) - IHC Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of colon, using Recombinant ARID1A Antibody (Ab089579).
Recombinant ARID1A Antibody (Ab089579) - IF/ICC Immunofluorescent analysis of SH-SY5Y cells, using Recombinant ARID1A Antibody (Ab089579).
Application
Application
Dilution info
IHC
1/100 - 1/200
WB
1/1000 - 1/2000
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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