Recombinant Canine CD16 Protein

Cat. No.: rp176255

GRADE & PURITY Animal Free, Carrier Free, Azide Free, His Tag, PBS Only, ≥95%(SDS-PAGE)

Expression System

HEK293

Accession #

E2RP87

Protein Tag

C-His

Expression system

HEK293

Endotoxin Concentration

<1.0 EU/μg

Overview

Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.

Description：

Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG) (By similarity).
Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger. Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation (By similarity).
Fc-binding subunit that associates with FCER1G adapters to form functional signaling complexes. Following the engagement of antigen-IgG complexes, triggers phosphorylation of immunoreceptor tyrosine-based activation motif (ITAM)-containing adapters with subsequent activation of phosphatidylinositol 3-kinase signaling and sustained elevation of intracellular calcium that ultimately drive NK cell activation (By similarity).
Mediates enhanced ADCC in response to afucosylated IgGs. 

Specifications

Product Name

Recombinant Canine CD16 Protein

Synonyms

Low affinity immunoglobulin gamma Fc region receptor III-A | IgG Fc receptor III-A | FcgammaRIIIA | CD16a

Grade

Animal Free, Azide Free, Carrier Free, His Tag, PBS Only

Specifications & Purity

Animal Free, Carrier Free, Azide Free, His Tag, PBS Only, ≥95%(SDS-PAGE)

Endotoxin Concentration

<1.0 EU/μg

Expression System

HEK293

Amino Acids

1-206 aa

Sequence

MWQLVSSTALLLLVSAGTQAADVPKAVVVLEPKWNRVLTMDSVTLKCQGDHLLRDNYTWLHNGRPISNQISTYIIKNASIKNSGEYRCQTDQSKLSDPVQLEVHTGWLLLQVPRLVFQEGELIQLKCHSWKNTPVRNVQYFQNGRGKKFFYNNSEYHIPAATSEHNGSYFCRGIIGKKNESSEAVNIIIQGSSLPSTSLLLSHWPQHHHHHH

Protein Tag

C-His

Accession #

E2RP87

Predicted molecular weight

22.0 kDa

SDS-PAGE

37.0-54.0 kDa, under reducing conditions; 37.0-54.0 kDa, under non-reducing conditions

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

SDS-PAGE: Recombinant Canine CD16 Protein (rp176255)

Recombinant Canine CD16 Protein (rp176255) - SDS-PAGE
2 μg/lane of Recombinant Canine CD16 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 37.0-54.0 kDa.