Recombinant Human SIRP beta 1/CD172b Protein

Cat. No.: rp183643

GRADE & PURITY Animal Free, Carrier Free, His Tag, PBS Only, ≥90%(SDS-PAGE)

Expression System

HEK293

Accession #

O00241-1

Protein Tag

C-His

Expression system

HEK293

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Testing in progress

Overview

Protein Function:
Signal-regulatory protein beta 1 (SIRP beta 1) is a disulfide-linked type I membrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily. The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions. Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains. The extracellular domain contains 3 intramolecular disulfide bonds and one interchain disulfide at Cys320. Positively charged residues within the transmembrane domain mediate interactions with DAP12 proteins which contain immunoreceptor tyrosine-based activation motifs (ITAMs). Proteins in the SIRP family are typically expressed in cells of monocyte, macrophage or dendritic lineages. Human SIRP beta 1 shares a 57% sequence identity with mouse and rat SIRP beta 1. SIRP beta 1 has a relatively short cytoplasmic region and lacks the signaling motifs for association with phosphatases. However, formation of the SIRP beta 1/DAP12 complex in myeloid cells induce tyrosine phosphorylation, mitogen-activated protein kinase activation, and cellular activation. Engagement of SIRP beta 1 by specific monoclonal antibodies promoted Fc gamma receptor-dependent or -independent phagocytosis in mouse peritoneal macrophages. Surfactant protein D (Sp-D) has been shown to bind SIRP alpha and SIRP beta 1 in a calcium-dependent and sugar-specific manner on a distinct binding site from CD47. Although the SIRP beta 1 extracellular regions share a high degree of homology with the SIRP alpha, SIRP beta 1 has been shown not to bind CD47.

Specifications

Product Name

Recombinant Human SIRP beta 1/CD172b Protein

Synonyms

CD172b antigen | CD172 antigen-like family member B | CD172b | DKFZp686A05192 | FLJ26614 | signal-regulatory protein beta 1 | signal-regulatory protein beta-1 | SIRP beta 1 | SIRPB1 | SIRP-beta-1 isoform 3 | SIRP-BETA-1

Grade

Animal Free, Carrier Free, His Tag, PBS Only

Specifications & Purity

Animal Free, Carrier Free, His Tag, PBS Only, ≥90%(SDS-PAGE)

Bioactivity

Testing in progress

Endotoxin Concentration

<1.0 EU/μg

Expression System

HEK293

Amino Acids

1-369 aa

Sequence

MPVPASWPHLPSPFLLMTLLLGRLTGVAGEDELQVIQPEKSVSVAAGESATLRCAMTSLIPVGPIMWFRGAGAGRELIYNQKEGHFPRVTTVSELTKRNNLDFSISISNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAVRATPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPAGDSVSYSIHSTARVVLTRGDVHSQVICEIAHITLQGDPLRGTANLSEAIRVPPTLEVTQQPMRAENQANVTCQVSNFYPRGLQLTWLENGNVSRTETASTLIENKDGTYNWMSWLLVNTCAHRDDVVLTCQVEHDGQQAVSKSYALEISAHQKEHGSDITHEAALAPTAHHHHHH

Protein Tag

C-His

Accession #

O00241-1

Predicted molecular weight

37.9 kDa

SDS-PAGE

42.1-54.8 kDa, under reducing conditions; 38.0-52.6 kDa, under non-reducing conditions.

Molecule Type

Protein

Storage and Shipping

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

SDS-PAGE: Recombinant Human SIRP beta 1/CD172b Protein (rp183643)

Recombinant Human SIRP beta 1/CD172b Protein (rp183643) - SDS-PAGE
3 μg/lane of Recombinant Human SIRP beta 1/CD172b Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 42.1-54.8 kDa under reducing conditions and 38.0-52.6 kDa under non-reducing conditions.