Recombinant Protein A/G, HRP conjugate

Cat. No.: rp303272

GRADE & PURITY BioReagent, Azide Free, High Performance, for Western blot, for ELISA, Suitable for Immunohistochemistry(IHC), 1.0 mg/mL

Accession #

P19909,P02976

Protein Tag

No tag

Expression system

E. coli

Overview

Aladdin offers recombinant protein A/G conjugated to our LumiDye™ AF Dyes (Table 1).

Table 1. Spectral characteristics of protein A/g conjugates.

Cat.No.	Conjugate	λmax (nm)	Em (nm
rp303272	HRP	NA	NA
rp303273	FITC	498	517
rp303275	AF488	490	525
rp303276	AF555	553	568
rp303277	AF594	590	618
rp303278	AF647	650	668
rp302416	Cy3	554	566

Aladdin recombinant Protein A/G is a genetically engineered protein containing 5 IgG-binding regions of protein A and 2 of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant A/G-Cys to ensure the maximum specific IgG binding. The recombinant Protein A/G is ideal for making affinity gel to purification of polyclonal or monoclonal IgG antibodies. Protein A/G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house, rabbit, guinea pig, pig, dog and cat.

We have detected the binding data of different IgG subtypes with recombinant protein A/G, as shown in the Graph1.

Graph 1. Protein A/G affinity to selected species.

Recombinant Protein A/G is covalently conjugated to HRP, followed by size‑exclusion chromatography to remove most unreacted proteins and free HRP, yielding a high‑quality conjugate. It is used for probing and antibody detection in Western blotting (WB), enzyme‑linked immunosorbent assay (ELISA), immunohistochemistry (IHC), Immunoprecipitation(IP) and other immunoassay protocols, and is especially suitable for IgG antibodies from a wide range of host species.

Specifications

Product Name

Recombinant Protein A/G, HRP conjugate

Synonyms

Protein A/G, horseradish peroxidase conjugated | Protein A/G-HRP | Protein A/G-Peroxidase | Horseradish Peroxidase-labeled Protein A/G | HRP-labeled Protein A/G | HRP-Protein A/G

Grade

Azide Free, BioReagent, for ELISA, for Western blot, High Performance, Suitable for Immunohistochemistry(IHC)

Specifications & Purity

BioReagent, Azide Free, High Performance, for western blot, for ELISA, Suitable for Immunohistochemistry(IHC), 1.0 mg/mL

Biochemical and Physiological Mechanisms

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immu

Amino Acids

35-330 aa & 373-497 aa

Sequence

NAAQHDEAQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNKEQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNKEQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNKEQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDSLEGSGSGTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGVWTYDDATKTFTVTE

Conjugation

HRP

Accession #

P19909, P02976

Note

Because staining protocols vary with application, the appropriate dilution of the protein A conjugates should be determined empirically.

Molecule Type

Protein

Storage and Shipping

Concentration

1.0 mg/mL

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at-20°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Western Blot: Recombinant Protein A/G, HRP conjugate (rp303272)

Recombinant Protein A/G, HRP conjugate (rp303272) - Western Blot 

All lanes: SDHA Mouse mAb (Ab126844) at 1/1000 dilution 

Samples: Lysates at 20 µg per lane 

Secondary: Recombinant Protein A/G, HRP conjugate (rp303272) at 1/20000 dilution 

Predicted band size: 70 kDa 

Observed band size: 66 kDa 

Exposure time: 69.8 s

Western Blot: Recombinant Protein A/G, HRP conjugate (rp303272)

Recombinant Protein A/G, HRP conjugate (rp303272) - Western Blot 

All lanes: Aldolase C Mouse mAb (Ab088122) at 1/1000 dilution 

Samples: Lysates at 20 µg per lane 

Secondary: Recombinant Protein A/G, HRP conjugate (rp303272) at 1/20000 dilution 

Predicted band size: 39 kDa 

Observed band size: 39 kDa 

Exposure time: 19.8 s

Application

• Application: Dilution info
• ELISA:

  1/5000-1/100000;

• WB:

  1/2000-1/20000

• IP:

  1:2000-1:20000