Recombinant SFT Antibody - Primary antibody, specific to UBE2D1, Rabbit IgG

Cat. No.: Ab127323

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.4 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human SFT Antibody, Recombinant, could be used for WB, IHC and so on.
Application:

WB: 1/500-1/1000
IHC: 1/50-1/100

IF/ICC: 1/200
Protein Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of CHIP, TRAF6 and TRIM63/MURF1. Ubiquitinates CHIP-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4. 

Specifications

Product Name

Recombinant SFT Antibody - Primary antibody, specific to UBE2D1, Rabbit IgG

Synonyms

E2(17)KB1 | SFT | Stimulator of Fe transport | UB2D1_HUMAN | UBC 4/5 | UBC4/5 | UBC4/5 homolog | UBCH 5 | UBCH 5A | UBCH5 | UBCH5A | Ube2d1 | Ubiquitin carrier protein | Ubiquitin carrier protein D1 | Ubiquitin conjugating enzyme E2 17 kDa 1 | Ubiquitin c

Specifications & Purity

ExactAb™, Validated, Recombinant, 0.4 mg/mL

Host species

Rabbit

Specificity

UBE2D1

Immunogen

A synthetic peptide derived from human SFT (AA 119-132)

Positive Control

WB: HeLa, Jurkat, A431, HEK293, RAW 264.7, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Antigen affinity purified

Concentration

0.4 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant SFT Antibody (Ab127323)

Recombinant SFT Antibody (Ab127323) - Western Blot
All lanes: Recombinant SFT Antibody (Ab127323) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 16 kDa
Exposure time: 3.0 s

IHC: Recombinant SFT Antibody (Ab127323)

Recombinant SFT Antibody (Ab127323) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant SFT Antibody (Ab127323). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Recombinant SFT Antibody (Ab127323)

Recombinant SFT Antibody (Ab127323) - IF/ICC
IF analysis of Recombinant SFT Antibody (Ab127323) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant SFT Antibody (Ab127323) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

Application

• Application: Dilution info
• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

• WB:

  1/500-1/1000 ;

Associated Targets(Human)

UBE2D1 Tbio Ubiquitin-conjugating enzyme E2 D1 (0 Activities)

Discover Target: UBE2D1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action