Rhodamine B isothiocyanate - mixture of isomers , CAS No.36877-69-7

CAS: 36877-69-7 Cat. No.: R105502 Molecular Weight: 536.08 EC Number: 253-248-9

GRADE & PURITY mixture of isomers

Synonyms

RBITC | 9-[2-Carboxy-5-isothiocyanatophenyl]-3,6-bis(diethylamino)xanthylium chloride

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Rhodamine B isothiocyanate (RBITC) is a fluorescent dye. It acts as an anterograde and retrograde marker of retinal neurons in the adult rat. (current GD)It is also known as RBITC, RhIc, RITC, and TRITC. 

Application：

Rhodamine B isothiocyanate has been used as a fluorescent probe to label the pathogenic bacterium Staphylococcus aureus and is also used to label type I collagen (C1). Other applications include -：labeling immunoglobulins；as a fluorescent probe of living cells；RBITC-Iabeled molecules like dextrans have been applied as probes of a cell′s endocytic activity and to mark cells to allow their subsequent migration to be detected

Specifications

Synonyms

RBITC | 9-[2-Carboxy-5-isothiocyanatophenyl]-3, 6-bis(diethylamino)xanthylium chloride

Specifications & Purity

mixture of isomers

Biochemical and Physiological Mechanisms

Rhodamine B isothiocyanate is a mixture of isomers for fluorescence applications including fluorescent marking of proteins (labelling efficiency with bovine serum albumin >70%). It is a derivative of phenolphtalein.

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• WGK Germany: 3
• UN Number: 3335
• Molecular Weight: 536.08

Chemical and Physical Properties

• Solubility: Soluble in methanol (1%), and water.
• Sensitivity: Light and Moisture sensitive

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69. Vincent Mukwaya, Xiaolei Yu, Shuo Yang, Stephen Mann, Hongjing Dou.  (2025)  Adaptive ATP-induced molecular condensation in membranized protocells.  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,  122  (13): (e2419507122).  [PMID:40127264] [10.1073/pnas.2419507122]

70. Mingchen Lv, Jiaxi Xu, Ran Chen, Wei Hu, Yuxiao Zhou, Min Sun, Zhen Fan, Jianzhong Du.  (2025)  Albumin Corona-Coated Nanoscale Metal–Organic Framework for Enzyme-Mediated Cascade Metabolization of Uric Acid in Hyperuricemia.  Small,      [PMID:40026041] [10.1002/smll.202412612]

71. Yang Jing, Wan Shi-yuan, Song Qiu-yi, Xie Yun-hao, Wan Jun, Zhou Yi-hao, Zhang Zi-tong, Xiao Yu-shuo, Li Xi, Chen Hong, Liu Xin-ran, Xu Li, You Hui-juan, Hu De-sheng, Petersen Robert B., Zhang Yong-hui, Zheng Ling, Zhang Yu, Huang Kun.  (2024)  Angiopoietin-like protein 8 directs DNA damage responses towards apoptosis by stabilizing PARP1-DNA condensates.  CELL DEATH AND DIFFERENTIATION,      [PMID:39592710] [10.1038/s41418-024-01422-2]

72. Wen Ping, Huang Hanwei, Zhang Ruizhe, Zheng Hanqi, Liang Tingxizi, Zhuang Chuyue, Wu Qing, Wang Junxia, Liu Feng, Zhang Ke, Wu Wei, He Kaixin, Liu Funan, Li Hongjun, Gu Zhen.  (2025)  Coacervate vesicles assembled by liquid–liquid phase separation improve delivery of biopharmaceuticals.  Nature Chemistry,      [PMID:39806140] [10.1038/s41557-024-01705-8]

73. Weijie Jiang, Xin Guan, Liang Wang, Yuqian Mao, Panfei Ma, Wei Liu, Yunxing Li, To Ngai, Hang Jiang.  (2025)  Fabrication of Porous Proteinaceous Microspheres via One-Step Pickering Double Emulsions: Controllable Structure and Interfacial Cascade Biocatalysis.  LANGMUIR,      [PMID:40094198] [10.1021/acs.langmuir.4c04555]

74. Chong Wang, Xinyuan Yang, Qiao Wang, Linyi Zhang, Luoran Shang.  (2024)  Glucose Responsive Coacervate Protocells from Microfluidics for Diabetic Wound Healing.  Advanced Science,      [PMID:38768125] [10.1002/advs.202400712]

75. Xiangyu Duan, Yongzhen Mi, Tingyu Lei, Xiu Yun Daphne Ma, Zhong Chen, Junhua Kong, Xuehong Lu.  (2025)  Highly Elastic Spongelike Hydrogels for Impedance-Based Multimodal Sensing.  ACS Nano,      [PMID:39761359] [10.1021/acsnano.4c16694]

76. Linwei Yu, Xi Li, Tianyi Shi, Ning Li, Donge Zhang, Xikai Liu, Yushuo Xiao, Xinran Liu, Robert B. Petersen, Weikang Xue, Yanxun V. Yu, De-sheng Hu, Li Xu, Hong Chen, Ling Zheng, Kun Huang, Anlin Peng.  (2025)  Identification of novel phenolic inhibitors from traditional Chinese medicine against toxic α-synuclein aggregation via regulating phase separation.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:39818366] [10.1016/j.ijbiomac.2025.139875]

77. Liang Tingxizi, Dong Yuxiang, Cheng Irina, Wen Ping, Li Fengqin, Liu Feng, Wu Qing, Ren En, Liu Peifeng, Li Hongjun, Gu Zhen.  (2024)  In situ formation of biomolecular condensates as intracellular drug reservoirs for augmenting chemotherapy.  Nature Biomedical Engineering,      [PMID:39271933] [10.1038/s41551-024-01254-y]

78. Liangqiang Chen, Mengdi Hao, Wanqiu Huang, Shaoning Yu, Hao Shen, Fan Yang, Li Wang, Huabin Tu.  (2024)  Integration of multienzyme co-immobilization and biomimetic catalysis in magnetic metal–organic framework nanoflowers for α-amylase detection in fermentation samples.  FOOD CHEMISTRY,      [PMID:38402762] [10.1016/j.foodchem.2024.138773]

79. Jian Liu, Junbo Li, Yan Huang, Tong Li, Cheng Xu, Zhengyu Tao, Wei Ji, Xin Huang.  (2025)  Liquid-to-gel transitions of phase-separated coacervate microdroplets enabled by endogenous enzymatic catalysis.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,      [PMID:40184654] [10.1016/j.jcis.2025.137486]

80. Chaochao Chen, Liang Luo, Jianzhong Shen, Zhanhui Wang, Yantong Pan.  (2024)  Metal–Organic Framework-Based Cascade Catalysis-Enabled Fluorescent ELISA with Higher Enzymatic Stability for Zearalenone Detection in Maize.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,      [PMID:39704662] [10.1021/acs.jafc.4c07146]

81. Shuting Zhang, Hong Zhou, Xuan Chen, Shasha Zhu, Dan Chen, Dan Luo, Siyuan Chen, Wei Liu.  (2024)  Microneedle Delivery Platform Integrated with Codelivery Nanoliposomes for Effective and Safe Androgenetic Alopecia Treatment.  ACS Applied Materials & Interfaces,      [PMID:38507687] [10.1021/acsami.3c16608]

82. Yingjie Du, Xiuyan Luo, Xiaohong Ye, Meijia Song, Yingjia Li, Shumao Yang, Caoxing Huang, Jiandong Cui.  (2025)  Molecular imprinted photoresponse hybrid biocatalyst for ultrasensitive glutamate detection.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:40147642] [10.1016/j.ijbiomac.2025.142540]

83. Hailong Liu, Ming Liu, Pengbo Zhang, Kangle Xue, Tongjie Yao, Li Liu, Yudong Huang.  (2024)  POSS-polyurethane prepolymer strengthened and toughened CF/epoxy resin composites for room and simulated Arctic ambient temperature.  POLYMER,      [PMID:] [10.1016/j.polymer.2024.126692]

84. Shuxian Zhang, Xiaofei Wang, Jili Han, Xiaoxuan Fan, Keshuai Liu, Lingling Yang, Hao Yang, Xiaofei Chen, Xueqin Ma, Guoning Chen.  (2025)  Precise Identification of Inhibitors for Coagulation Reactions from Complex Extracts through Monitoring of Biological Aggregates Combined with a Targeted Fishing Technique.  ANALYTICAL CHEMISTRY,      [PMID:40073066] [10.1021/acs.analchem.4c07092]

85. Miaoyu Gan, Ailing Cao, Luyun Cai, Xia Xiang, Jian Li, Qian Luan.  (2024)  Preparation of cellulose-based nanoparticles via electrostatic self-assembly for the pH-responsive delivery of astaxanthin.  FOOD CHEMISTRY,      [PMID:39321653] [10.1016/j.foodchem.2024.141324]

86. Yongming Wang, Yangcan Ming, Zhichao Yu, Zhenjin Xu, Minglang Zou, Cuiping Chen, Fang Luo, Da Huang, Na Wang, Zhenyu Lin, Zuquan Weng.  (2025)  ROS-Responsive Cationic Polymers with Intrinsic Anti-Inflammatory Activity for Intracellular Protein Delivery.  BIOMACROMOLECULES,      [PMID:40114391] [10.1021/acs.biomac.4c01593]

87. Li Xi, Yu Linwei, Liu Xikai, Shi Tianyi, Zhang Yu, Xiao Yushuo, Wang Chen, Song Liangliang, Li Ning, Liu Xinran, Chen Yuchen, Petersen Robert B., Cheng Xiang, Xue Weikang, Yu Yanxun V., Xu Li, Zheng Ling, Chen Hong, Huang Kun.  (2024)  β-synuclein regulates the phase transitions and amyloid conversion of α-synuclein.  Nature Communications,  15  (1): (1-17).  [PMID:39384788] [10.1038/s41467-024-53086-8]

88. Qianjin Li, Rui Li, Mengdi Wang, Jinjin Wang, Tingting Wang, Yu Jin, Jianlin Li, Fenying Wang.  (2025)  Development of fluorescent artificial receptors for specific recognition and rapid detection of Escherichia coli O157:H7.  FOOD CHEMISTRY,      [PMID:40651138] [10.1016/j.foodchem.2025.145507]

89. Zhuo Zhang, Mingchen Lv, Jiaxi Xu, Yaping Liu, Jinlong Qin, Zhen Fan, Jianzhong Du.  (2025)  A Metal-Peptide Framework as a Nanozyme for the Attenuation of Amyloid-β Aggregation and Reactive Oxygen Species.  JACS Au,      [PMID:41001643] [10.1021/jacsau.5c00721]

90. Kaixi Cui, Tianzheng Li, Yifei Ma, Chuanjin Zhang, Ke Zhang, Chao Qi, Kaiyong Cai.  (2025)  Ultrasound-Responsive Drug Delivery System Based on Piezoelectric Catalytic Mechanisms.  Journal of Functional Biomaterials,  16  (8): (304).  [PMID:40863324] [10.3390/jfb16080304]

91. Fenying Wang, Shuqing Xiong, Jinjin Wang, Haoqi Jia, Kai Gao, Jianlin Li, Hua Guo, Qianjin Li.  (2025)  Principal component analysis-assisted fluorescent phenylboronic acid probes for discrimination and detection of living pathogenic bacteria.  Analytical Methods,      [PMID:41070918] [10.1039/D5AY01421B]

92. Tianrong Wang, Tiantian Chen, Jing Ming, Qingbo Jiao, Jiayi Han, Hao Li, Zhijun Song, Gerui Ren, Min Huang, Qunfang Lei, Wenjun Fang, Zexing Cao, Khaled F. El-Massry, Hujun Xie.  (2025)  Phase behaviour of lactoferrin and β-lactoglobulin complexes: Revealing mechanisms from an integrated experimental and theoretical perspective.  FOOD HYDROCOLLOIDS,      [PMID:] [10.1016/j.foodhyd.2025.111778]

93. Chenxiao Chu, Mingli Wei, Che Bian, Xiaoshuang Bi, Yaxin Deng, Peifu Xiao, Jiansong Zhao, Yuying Wang, Haibing He, Jingxin Gou, Tian Yin, Xing Tang, Li Yang, Hongbo Zhang, Yu Zhang.  (2025)  Oral Dosed Organo-Silica Nanoparticles Restore Glucose Homeostasis and β-Cell Function in Diabetes Rats.  ADVANCED FUNCTIONAL MATERIALS,      [PMID:] [10.1002/adfm.202519628]

94. Hao Wang, Ruisi Cai, Shenqiang Wang, Yinxian Yang, Tao Sheng, Wentao Zhang, Shiqi Wang, Jiahuan You, Ziyi Lu, Kangfan Ji, Yunlong Jiao, Mengyuan Ma, Nianou Wang, Ke Li, Wenjin Chu, Zhixi Yang, Jicheng Yu, Yuqi Zhang, Zhen Gu.  (2025)  A wearable transdermal device for on-demand drug delivery.  Matter,      [PMID:] [10.1016/j.matt.2025.102040]

95. Wang Huaimin, Mao Duo, Wang Youzhi, Wang Kai, Yi Xiaoyong, Kong Deling, Yang Zhimou, Liu Qian, Ding Dan.  (2015)  Biocompatible fluorescent supramolecular nanofibrous hydrogel for long-term cell tracking and tumor imaging applications.  Scientific Reports,  5  (1): (1-10).  [PMID:26573372] [10.1038/srep16680]

96. Jizhou Li, Shiruoyu Zhou, Ruining Bai, Zhifeng Fu.  (2025)  A 3D-printed integrated maneuverable device for sensitive colorimetry of Pseudomonas aeruginosa.  JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,      [PMID:40315595] [10.1016/j.jpba.2025.116934]

97. Li Wen, Jinxia Zheng, Zhijie Ke, Jian Dong.  (2025)  Macromolecular crowding effect on the β-galactosidase cascade reaction under multivalency and chain overlapping conditions of glycopolymers.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:40389007] [10.1016/j.ijbiomac.2025.144343]

98. Yuling Wang, Jun Deng, Xi Wang, Dan Luo, Shujing Ren, Xueping Guo, Dan Chen, Rui Liu, Siyuan Chen, Wei Liu.  (2025)  Mini Hyaluronic Acid-modified Nanoliposomes for Topical Co-Delivery of Paeonol, Totarol and Ergothioneine: A Synergistic Adjuvant Therapy for Acne.  JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY,      [PMID:] [10.1016/j.jddst.2025.107648]

99. Wenkui Mo, Zeyu Liang, Bo Zhang, Lin Xiao, Xun Liu, Zhigao Yi, Shangzhi Xie, Qiyue Wang, Liang Shi, Taobo Luo, Feng Li, Leilei Wu, Yuanzhe Piao, Fangyuan Li, Jian Zeng, Daishun Ling.  (2025)  A targeted antiferromagnetic nanoprobe for ultra-sensitive magnetic resonance imaging detection of sub-millimeter esophageal tumors.  BMEMat,      [PMID:] [10.1002/bmm2.70040]

100. Xinqiuyue Wang, Lei Zhang, Jiajia Su, Haokun Xue, Han Zhou, Fanglei Xue, Yajie Wang, Danyi Quan, Jianping Liu, Wenli Zhang.  (2026)  Eradicating Helicobacter Pylori with a Multifunctional Trojan Horse Nanoplatform Armored with β-Cyclodextrin.  ADVANCED FUNCTIONAL MATERIALS,      [PMID:] [10.1002/adfm.202528258]

101. Leilei Zhang, Haoheng Bai, Weiqi Sun, Luqi Wang, Minyan Duan, Qianying Liu, Chao Zhou, Xueyang Fang, Tao Zhang.  (2026)  NIR-II Photoacoustic-Cavitation Boosted Metabolic Reprogramming Regulation Toward the Localized TNBC Growth Inhibition.  ACS Applied Materials & Interfaces,      [PMID:] [10.1021/acsami.6c02884]

102. Didi Liu, Xiangyu Jin, Yao Zeng, Xinru Zhou, Minyu Wang, Hui Shen, Yi Huang.  (2026)  Bafetinib enhances anti-tumor immunity by activating the NLRP3 inflammasome in macrophage.  Autophagy,      [PMID:41906654] [10.1080/15548627.2026.2653011]

103. Hou Bo, Ma Dewei, Yang Keke, Peng Mingwei, Liu Qinglong, Heng Ziwen, Chen Siyuan, Liu Wei.  (2026)  Co-delivering Retinol and Niacinamide via Liposomes: Enhanced Stability, Efficient Skin Permeation, and Improved Anti-Aging/Skin-Brightening Efficacy.  Journal of Pharmaceutical Innovation,  21  (4): (340).  [PMID:] [10.1007/s12247-026-10594-y]

104. Peng Ziling, Wang Xiu, Tan Fatang, Zhou Xian, Fan Zeyu, Lu Qi, Chen Xia, Shi Yu, Xia Yu.  (2026)  Facile Fabrication of N-Doped Graphitic Carbon-Modified g-C3N4 with Enhanced Photocatalysis Performance Under Visible Light Irradiation.  WATER AIR AND SOIL POLLUTION,  237  (13): (779).  [PMID:] [10.1007/s11270-026-09461-w]

105. Turong Shi, Jiachun Feng, Beibei Tang.  (2026)  Synergistic metal-MOF coordination and assisted etching enabled high-flux nanofiltration membrane for superior dye/salt separation.  JOURNAL OF MEMBRANE SCIENCE,      [PMID:] [10.1016/j.memsci.2026.125490]

106. Yinuo Jin, Ting Zhang, Meng Sun, Yulu Li, Yutong Zhang, Zhaohui Yan, Jingbo Liu, Dongyan Chen, Xuanting Liu.  (2026)  Targeted design and controllable assembly of egg white protein complexes guided by phase behavior: Thermal stability and processing properties of ovotransferrin-lysozyme heteroprotein complexes.  FOOD RESEARCH INTERNATIONAL,      [PMID:] [10.1016/j.foodres.2026.118891]

107. Yize Zhao, Nan Wang, Chengwu Cai, Jiayi Ding, Mingqi Huang, Jingting Zhang, Zhenni Li, Ning Li.  (2026)  Effect of psyllium husk gel addition on the quality of whole wheat steamed bread: insights from rheological properties and protein structural changes.  FOOD CHEMISTRY,      [PMID:] [10.1016/j.foodchem.2026.149692]

108. Ma Tianyu, Li Qiang, Chen Xingwu, Dong Jixiang, Jiang Yuqing, Li Shanshan.  (2026)  Hydrogel microneedle delivery of a palmitoylated peptide promotes hair regeneration via dual activation of Wnt/β-catenin and cAMP–PKA signaling.  JOURNAL OF NANOBIOTECHNOLOGY,      [PMID:] [10.1186/s12951-026-04542-8]