10mM in DMSO for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 28 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Information
5-Hydroxytryptophan (5-HTP, NSC-92523), also known as oxitriptan (INN), is a naturally occurring amino acid and chemical precursor for the treatment of depression.
In vitro
5-Hydroxytryptophan (5-HTP) is the intermediate metabolite of the essential amino acid L-tryptophan (LT) in the biosynthesis of serotonin. It easily crosses the blood-brain barrier and effectively increases central nervous system (CNS) synthesis of serotonin. Therapeutic administration of 5-HTP has been shown to be effective in treating a wide variety of conditions, including depression, fibromyalgia, binge eating associated with obesity, chronic headaches, and insomnia. Clinical available evidence suggests 5-Hydroxytryptophan is better than placebo at alleviating depression.
In vivo
LD50: Mice 1800mg/kg (i.g.); Rats 300mg/kg (i.g.)
Cell Data
cell lines：Leukemia, non-small cell lung cancer, colon cancer, central nervous system cancer, melanoma, ovarian cancer, renal cancer, prostate cancer, breast cancer
Concentrations：
Incubation Time：
Powder Purity:≥99%
| Isomeric SMILES | C1=CC2=C(C=C1O)C(=CN2)CC(C(=O)O)N |
|---|---|
| Molecular Weight | 220.23 |
| Beilstein | 88199 |
| Reaxy-Rn | 88199 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=88199&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Melt Point(°C) | 298-300 °C |
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| 1. Wenting Lin, Kan Li, Yingjun Qin, Xing Han, Xiaohui Chen, Yuan Ren. (2023) Flunitrazepam induces neurotoxicity in zebrafish through microbiota-gut-brain axis. SCIENCE OF THE TOTAL ENVIRONMENT, [PMID:37532048] [10.1016/j.scitotenv.2023.165974] |
| 2. Pan Hu, Xin Yan, Yijia Zeng, Zherui Jiang, Juan Liu, Wu-wen Feng. (2023) An UPLC-MS/MS method for targeted analysis of microbial and host tryptophan metabolism after administration of polysaccharides from Atractylodes macrocephala Koidz. in ulcerative colitis mice. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, [PMID:37523866] [10.1016/j.jpba.2023.115585] |
| 3. Wang Yingying, Chen Xueman, Chen Qiaoyu, Zhou Ning, Wang Xin, Zhang Alei, Chen Kequan, Ouyang Pingkai. (2022) Construction of cell factory capable of efficiently converting l-tryptophan into 5-hydroxytryptamine. Microbial Cell Factories, 21 (1): (1-9). [PMID:35331215] [10.1186/s12934-022-01745-0] |
| 4. Lvqi Zhou, Ning Hao, Xiaotian Li, Jiaxi Chen, Runyu Yang, Changqin Song, Yang Sun, Qi Zhang. (2020) Nattokinase mitigated dextran sulfate sodium-induced chronic colitis by regulating microbiota and suppressing tryptophan metabolism via inhibiting IDO-1. Journal of Functional Foods, [PMID:] [10.1016/j.jff.2020.104251] |
| 5. Zhao Shiyuan, Yu Tao, Du Yingxiang, Sun Xiaodong, Feng Zijie, Ma Xiaofei, Ding Wen, Chen Cheng. (2019) An organic polymer monolith modified with an amino acid ionic liquid and graphene oxide for use in capillary electrochromatography: application to the separation of amino acids, β-blockers, and nucleotides. MICROCHIMICA ACTA, 186 (9): (1-10). [PMID:31432257] [10.1007/s00604-019-3723-z] |
| 6. Yamin Liu, Xunjiang Wang, Jiaojiao Wei, Kangning Fu, Yilin Chen, Linnan Li, Zhengtao Wang, Li Yang. (2024) Comprehensive Profiling of Amino Acids and Derivatives in Biological Samples: A Robust UHPLC-MS/MS Method for Investigating Acute Lung Injury. JOURNAL OF CHROMATOGRAPHY A, [PMID:38537486] [10.1016/j.chroma.2024.464816] |
| 7. Haoming Guo, Nan Xiao, Yabo Liang, Xueke Liu, Peng Wang, Donghui Liu. (2025) Neurotoxic impacts of neonicotinoid pesticide residues in Chinese honey on Apis mellifera L.. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, [PMID:40879281] [10.1002/jsfa.70154] |
| 8. Shikai Zhang, Qing Xu, Changbo Ji, Xiaoyu Han, Yang Zhou, Chao Liang, Linran Ma, Weijian Sun, Yanling Li, Zhengyou Yang, Fengchun Zhao, Yuan Tian. (2023) Study on secondary metabolites of endophytic fungus Diaporthe sp. AC1 induced by tryptophan analogs. Frontiers in Microbiology, [PMID:37876783] [10.3389/fmicb.2023.1254609] |
| 9. Jin Yuan Zhang, Ying Zhang, Yu Zou, Ze Li Bo Xu, Bo Zhang, Wang Ren. (2023) Physicochemically modulated fluorescence-scattering ratiometric sensor for selective and visual detection of levodopa. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, [PMID:38091648] [10.1016/j.saa.2023.123746] |
| 10. BingBing Wang, Shuai Liu, HaiDe Wang, JianZhong Xu, WeiGuo Zhang. (2023) Efficient biosynthesis of high-value 5‑Hydroxytryptophan using a multienzyme cascade. Molecular Catalysis, [PMID:] [10.1016/j.mcat.2023.113274] |
| 11. Yan Wang, Bin Peng, Yuanyuan Gong, Yingrong Xie, Tingting Feng, Xiaolin Liu, Bowei Chen, Yong Zhang. (2023) Carboxyl and amino acid functionalized indole-based polymer for ultrafast uranium extraction in aqueous solution. JOURNAL OF MOLECULAR LIQUIDS, [PMID:] [10.1016/j.molliq.2023.122143] |
| 12. Qixing Nie, Yonggan Sun, Mingzhi Li, Sheng Zuo, Chunhua Chen, Qiongni Lin, Shaoping Nie. (2023) Targeted modification of gut microbiota and related metabolites via dietary fiber. CARBOHYDRATE POLYMERS, [PMID:37321707] [10.1016/j.carbpol.2023.120986] |
| 13. Ya-Qian Wang, Li Li, Jin Yin, Xu Yu, Xiaowei Wu, Li Xu. (2023) Turn on fluorescence detection of curcumin in food matrices by the novel fluorescence sensitizer. ANALYTICA CHIMICA ACTA, [PMID:37005020] [10.1016/j.aca.2023.341094] |
| 14. Feifei Song, Tao Gu, Lin Zhang, Jiaxing Zhang, Shengping You, Wei Qi, Rongxin Su. (2023) Rational design of tryptophan hydroxylation 1 for improving 5-Hydroxytryptophan production. ENZYME AND MICROBIAL TECHNOLOGY, [PMID:36736156] [10.1016/j.enzmictec.2023.110198] |
| 15. Zhuangzhuang Li, Baoyan Ding, Mustafa R. K. Ali, Lizhen Zhao, Xiaoling Zang, Zhihua Lv. (2022) Dual Effect of Tryptamine on Prostate Cancer Cell Growth Regulation: A Pilot Study. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23 (19): (11087). [PMID:36232383] [10.3390/ijms231911087] |
| 16. Wu Lijuan, Ran Lisha, Wu Yazeng, Liang Manyu, Zeng Jing, Ke Famin, Wang Fang, Yang Jian, Lao Xiaoqing, Liu Li, Wang Qin, Gao Xiaowei. (2022) Oral Administration of 5-Hydroxytryptophan Restores Gut Microbiota Dysbiosis in a Mouse Model of Depression. Frontiers in Microbiology, [PMID:35572711] [10.3389/fmicb.2022.864571] |
| 17. Wang Yan, Liu Xiaolin, Xie Yingrong, Chen Bowei, Zhang Yong. (2022) Effective and rapid adsorption of uranium via synergy of complexation and cation-π interaction. JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, 331 (2): (1115-1126). [PMID:] [10.1007/s10967-021-08179-9] |
| 18. Jingsheng Tian, Li Qin, Dongdong Li, Sinan Qin, Junhua Han, Wenhui Gao, Yingmin Jia. (2021) Carbofuran-imprinted sensor based on a modified electrode and prepared via combined multiple technologies: Preparation process, performance evaluation, and application. ELECTROCHIMICA ACTA, [PMID:] [10.1016/j.electacta.2021.139600] |
| 19. Zhang Yanfeng, He Yongzhi, Zhang Nan, Gan JiaJia, Zhang Shan, Dong Zhiyang. (2021) Combining protein and metabolic engineering strategies for biosynthesis of melatonin in Escherichia coli. Microbial Cell Factories, 20 (1): (1-13). [PMID:34454478] [10.1186/s12934-021-01662-8] |
| 20. Yuanyuan Xia, Yuan Wang, Meng Zhang, Faqiong Zhao, Baizhao Zeng. (2020) A highly selective electrochemical sensor based on surface molecularly imprinted copolymer for the detection of 5-hydroxytryptamine. MICROCHEMICAL JOURNAL, [PMID:] [10.1016/j.microc.2020.105748] |
| 21. Xu Da, Fang Mengjun, Wang Haijiao, Huang Lei, Xu Qinyang, Xu Zhinan. (2020) Enhanced production of 5-hydroxytryptophan through the regulation of L-tryptophan biosynthetic pathway. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 104 (6): (2481-2488). [PMID:32006050] [10.1007/s00253-020-10371-y] |
| 22. Guojun Weng, Yao Feng, Jing Zhao, Jianjun Li, Jian Zhu, Junwu Zhao. (2020) Sensitive detection of choline in infant formulas by SERS marker transformation occurring on a filter-based flexible substrate. SENSORS AND ACTUATORS B-CHEMICAL, [PMID:] [10.1016/j.snb.2020.127754] |
| 23. Lu Chen, Hui-Ting Lian, Xiang-Ying Sun, Bin Liu. (2017) Sensitive detection of L-5-hydroxytryptophan based on molecularly imprinted polymers with graphene amplification. ANALYTICAL BIOCHEMISTRY, [PMID:28327452] [10.1016/j.ab.2017.03.017] |
| 24. Qing Wang, Yao Long, Lin Yao, Li Xu, Zhi-Guo Shi, Lanying Xu. (2015) Preparation, characterization and application of a reversed phase liquid chromatography/hydrophilic interaction chromatography mixed-mode C18-DTT stationary phase. TALANTA, [PMID:26695288] [10.1016/j.talanta.2015.09.009] |
| 25. Lu Feng, Li Limao, Shen Kang, Qian Yayun, Zhang Pengfei, Yang Yan, Zhu Qunshan, Huang Yong, Yan Chunxiang, Wei Wei. (2024) An enzymatic reaction-based SERS saliva analysis microporous array chip for chiral differentiation and high-throughput detection of D-amino acids. MICROCHIMICA ACTA, 191 (11): (1-11). [PMID:39375224] [10.1007/s00604-024-06733-2] |
| 26. Huiyu Yang, Xinrui Zhang, Yang Gao, Zhuang Peng, Bo Su, Kai Li, Cunlin Zhang. (2025) Detection of melatonin and 5-HTP in dietary supplements based on multiple spectra. Frontiers in Nutrition, [PMID:39935577] [10.3389/fnut.2025.1532092] |
| 27. Ai Yulin, Huang Yusong, Zhao Hongru, Su Bingmei, Lin Juan. (2025) Engineered phenylalanine hydroxylase coupled with an effective cofactor synthesis and regeneration system for high-yield production of 5-hydroxytryptophan. Bioresources and Bioprocessing, 12 (1): (1-14). [PMID:40047997] [10.1186/s40643-025-00846-z] |
| 28. Feng Chen, Xiaoxia Yang, Yayun Tan, Huanhuan Li, Yan Yan, Xiang Zhao, Chengfeng Qiu. (2025) Targeted LC-MS/MS metabolomics reveals distinctive tryptophan pathway signatures in pneumocystis jirovecii pneumonia. CLINICA CHIMICA ACTA, [PMID:41232641] [10.1016/j.cca.2025.120716] |
