Acetylcholinesterase (ACHE), CAS No.9000-81-1

CAS: 9000-81-1 Cat. No.: A1520586 EC Number: 232-559-3

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Electrophorus electricus (Electric eel); ≥200 U/g enzyme powder

Bioactivity

≥200 U/g enzyme powder

Overview

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines. 

Molecular Weight: 280 kDa

Isoelectric Point: 5.5

Extinction Coefficient: E1% = 18.0 (280 nm) 

Application

The enzyme has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity. The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride.

Biochem/physiol Actions

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.

Specifications

Product Name

Acetylcholinesterase (ACHE), CAS No.9000-81-1

Synonyms

AChE | Acetylcholine acetylhydrolase | Cholinesterase, Acetyl | acetylcholinesterase | choline esterase I | cholinesterase | true cholinesterase

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Electrophorus electricus (Electric eel); ≥200 U/g enzyme powder

Biochemical and Physiological Mechanisms

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.

Bioactivity

≥200 U/g enzyme powder

CAS

9000-81-1

Enzyme Commission Number

3.1.1.7

Molecule Type

Enzyme

Storage and Shipping

Concentration

from Electrophorus electricus (Electric eel); ≥200 U/g enzyme powder

Reconstitution

Reconstitute in water or phosphate buffer at pH 8.0.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (12 months). Upon reconstitution, it is recommended to aliquot.

Unit definition

Under the conditions of 37°C, 0.1mol/L pH=8.0 phosphate buffer, 1μmol of iodide is decomposed per minute. The enzyme amount of acetylcholinesterase (ATCI) is one unit of acetylcholinesterase activity

References

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