Collagenase from Clostridium histolyticum(Type 1), CAS No.9001-12-1

CAS: 9001-12-1 Cat. No.: C128702 EC Number: 232-582-9 PubChem CID: 44384985

GRADE & PURITY EnzymoPure™, ≥125 units/mg dry weight

Accession #

P04180

Overview

Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4.

Type 1 crude collagenase has the original balance of collagenase, caseinase, clostripain and tryptic activities.
This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.

Specifications

Product Name

Collagenase from Clostridium histolyticum(Type 1), CAS No.9001-12-1

Synonyms

Collagenase I

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, ≥125 units/mg dry weight

Accession #

P04180

CAS

9001-12-1

Enzyme Commission Number

EC: 3.4.24.3

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥125 units/mg dry weight

Storage

Store at 2-8°C

Shipped In

Wet ice

Unit definition

One Unit releases one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5.

Associated Targets(Human)

HT-29 (80576 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MCF7 (126967 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SK-MEL-5 (47095 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

A549 (127892 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

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