Tissue dissociation for single-cell preparation is a critical methodological step linking the original sample state to downstream flow sorting, single-cell transcriptomics, spatial validation, and functional assays.
CCK-8 (Cell Counting Kit-8) is a colorimetric cell-viability assay system based on the water-soluble tetrazolium salt WST-8. It is widely used for cell proliferation, cytotoxicity, and drug-sensitivity evaluation.
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