Animal model of ovarian hyperstimulation syndrome
Animal model of ovarian hyperstimulation syndrome
Ovarian hyperstimulation syndrome (OHSS) is a common complication that occurs in women during superovulation, which may be life-threatening in severe cases and is a major cause of morbidity and mortality in assisted reproduction, with an overall incidence of 5% to 10% and a heavy incidence of 0.3% to 5%.
Operation method
Animal model of ovarian hyperstimulation syndrome
Materials and Instruments
Laboratory animals: Move The basic process of animal modeling of ovarian hyperstimulation syndrome can be divided into the following steps: Caveat OHSS model animals mainly exhibit elevated estrogen levels, overexpression of vascular endothelial growth factor (VEGF), acute increase in capillary permeability, massive extravasation of body fluids with secondary pleural effusion, ascites, and other organ function changes. With only substantial follicular development of the ovary if ovulation does not occur, sex hormones can decline rapidly without causing severe OHSS, and if luteal support with veterinary chorionic gonadotropin or hCC injections is continued after ovulation has been induced, estrogen levels can remain high, exacerbating the severity of OHSS. For more product details, please visit Aladdin Scientific website.
(1) Sexually immature (22 to -25 days old) or sexually mature rats;
② Sexually mature 4-week-old mice;
(iii) New Zealand white rabbits;
Adult female monkeys with regular menstrual cycles.
Experimental reagents:
① Pregnant mare serum gonadotrophin (PMSC);
② hCG;
③ Veterinary chorionic gonadotropin (HMC);
④ Follicle stimulating hormone;
⑤ Gonadotropin-releasing hormone antagonist.
A. Mouse model. Sexually mature 4 weeks old mice. Pregnant mare serum gonadotrophin (PMSC) 20 U was injected intraperitoneally once a day for 3 days to promote follicular development, and hCG 10 U or veterinary chorionic gonadotropin 10 U was given intraperitoneally on the 4th day to induce ovulation, and the number of ovulated eggs could be examined after 12 hours, and the ovarian volume and weight, thoracic and abdominal fluid accumulation, etc., could be observed after 24-48 hours. After 24-48 hours, the volume and weight of ovaries, pleural and abdominal fluid can be observed.
B. Rat model. The rat model can be performed in sexually immature (22-25 days old) or sexually mature rats, and the dose of the drug is varied accordingly due to the difference in body weight. In immature rats, PMSC 10-30 U was injected intraperitoneally once a day for 4 days, and hCG 10-30 U was injected intraperitoneally on the 5th day. The number of ovulated eggs could be checked after 17-20 hours, and the vascular permeability and VECF could be checked after 48 hours, and the weight of the ovary could be about 30 g after hCG injection for 48 hours. In sexually mature rats, PMSG was increased to 30-50 U for intraperitoneal injection for 4 days, and hCG was increased to 30-50 U for intraperitoneal injection accordingly.
C. Rabbit model. The OHSS model of adult New Zealand White rabbits can be administered by various methods: single-dose method, PMSG 200 U was injected intramuscularly in a single dose, and hCG 100 U was given on the 3rd day after injection to induce ovulation; continuous method, PMSC or human menopausal gonadotropin (HMC) 37.5～75 U was injected intramuscularly once a day for 6～7 days, and hCG was increased to 30～50 U intraperitoneally on the 7th day, and then hCG was increased to 30～50 U intraperitoneally accordingly. ～On the 7th day, hCC 2500~5000 U intramuscularly to induce ovulation; in the incremental method, HMC 75 U intramuscularly once a day for 3 days, HMG 150 U intramuscularly once a day for another 3 days, and hCG 2500~5000 U on the 7th day to induce ovulation. hCG can be examined in the thoracic and abdominal cavities after 3 days of hCG injection, and it can be observed that the pleural effusion, the volume of ascites, and the weight of ovary is more than 4 times that of the control ovary. The weight of the ovary was more than 4 times that of the control ovary, and after paraffin embedding, the number of corpus luteum was estimated to be more than 20 per ovary, which was an ideal OHSS model. The rabbit model is ideal for OHSS. Blood can be collected from the vein at the ear margin, which is more helpful for observing the changes of sex hormones and other related factors.
D. Female rhesus monkey rhesus monkey model. In this model, human ovulation regimen can be fully simulated, such as the controlled superovulation regimen, adult female monkeys with regular menstrual cycles were selected, and FSH 30 U was given intramuscularly on the first day of menstruation, 3 times a day, repeated for 6 days, followed by follicle stimulating hormone 30 U and gonadotropin 30 U intramuscularly, 3 times a day, continued for 2~3 days, and gonadotropin-releasing hormone antagonist 0.25 mg was given routinely. Subcutaneous injection was given once a day to control the endogenous LH peak. Ultrasound was performed every 1-2 days to monitor the follicular development, and according to the ultrasound results, hCG 1000-3000 U was given to promote ovulation, and the eggs could be retrieved after 27 hours, and hCG 500 U was given every two days for the luteal function support.