Animal model of pulmonary granulomatous vasculitis
Animal model of pulmonary granulomatous vasculitis
Pulmonary granulomatous vasculitis (pulmonary granulomatous vasculitis) is rare in clinical practice, and the exact etiology of the disease is still unclear. It may be related to cellular immunity, as immune complex deposition in the lesion tissue is rare, but there is often an increase in complement. When sensitized lymphocytes are re-exposed to antigens, they can activate monocytes and transform them into macrophages or histiocytes and multinucleated giant cells, which are involved in granuloma formation. At present, there are 1 main methods for the preparation of animal models of pulmonary granulomatous vasculitis: the establishment of animal models of pulmonary granulomatous vasculitis.
Operation method
Establishment of an animal model of pulmonary granulomatous vasculitis
Principle
Glucan is the polysaccharide portion of brewer's yeast cell shine, which can stimulate the monocyte-phagocyte system and cause the proliferation of macrophages, which can significantly improve the humoral and cellular immunity.
Materials and Instruments
Equipments: syringes, common surgical equipments, optical microscope Move The process of establishing an animal model of pulmonary granulomatous vasculitis generally involves the following steps: For more product details, please visit Aladdin Scientific website.
Reagent: Dextran
B. After a single injection of dextran, the animals rapidly developed pulmonary lesions, and light microscopy revealed the formation of scattered granulomatous lesions around blood vessels, predominantly in the periphery of the pulmonary arteries and small pulmonary arterioles, with occasional involvement of the alveolar capillaries and small veins.
C. A common phenomenon was the occlusion of the larger C. It is common for large vascular structures to be occluded by large typical epithelioid cells; fibrinoid degeneration is absent from these lesions, but focal necrosis and stenosis of the vessels are evident.
D. These granulomas are characteristic of "allergic" granulomas rather than foreign body reactions, and many of them contain large numbers of lymphocytes and typical epithelioid cells.