Animal model of zinc deficiency
Animal model of zinc deficiency
Zinc deficiency animal model, is the zinc deficiency of the animal organism, mainly manifested as poor feeding, slow growth and development, low immune function. At present, the establishment of zinc deficiency animal model is to give animals zinc deficiency feed.
Principle
The basic principle of zinc deficiency animal model is that after giving zinc-deficient feed to animals, zinc will be depleted in the animal's body, showing reduced food intake, slow weight gain, and with the prolongation of zinc deficiency, a series of symptoms consistent with human zinc deficiency, such as fur changes, bleeding, anemia, diarrhea and susceptibility to infection and death.
Appliance
Zinc deficiency animal models are mainly used for research on the effects of zinc deficiency on growth and immune function.
Operation method
Establishment of an animal model of zinc deficiency
Principle
The basic principle of zinc deficiency animal model is that after giving zinc-deficient feed to animals, zinc will be depleted in the animal's body, showing reduced food intake, slow weight gain, and with the prolongation of zinc deficiency, a series of symptoms consistent with human zinc deficiency, such as fur changes, bleeding, anemia, diarrhea and susceptibility to infection and death.
Materials and Instruments
Laboratory animal: Wistar rat Move The zinc deficiency animal model was modeled as follows: A, Male pure line Wistar rats weaned for 1-3 days were fed zinc-deficient diets for 48 weeks with strict control of zinc contamination in feed, drinking water and rearing environment. B, The animal room was clean and dust-free, the room temperature was about 25 ℃, and the light, day and night were artificially controlled for 12 hours each. For more product details, please visit Aladdin Scientific website.
Reagents:
① Zinc deficiency feed: sucrose 6500 g, purified soy protein 1900 g (dezincified by 0.5 % EDTA purified water), vegetable oil 1000 g, mixed salt 584 g, multivitamin 16 g