Protocols

Brilliant Green Agar w/Sulfadiazine (Powder)

Directions per Liter:

Dissolve 58grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).


Component g/liter
Agar 20
Lactose 10
Sucrose 10
Pancreatic Digest of Casein 5
Peptic Digest of Animal Tissue 5
Sodium Chloride 5
Yeast Extract 3
Phenol Red 0.08
Sulfadiazine 0.08
Brilliant Green 0.0125
Total: 58.1725g/Liter


Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Categories: Protocols

Da — when not otherwise indicated, molecular weight units are daltons.   Mw — weight-average molecular weight.   Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Cite this article

Aladdin Scientific. "Brilliant Green Agar w/Sulfadiazine (Powder)" Aladdin Knowledge Base, updated Jul 27, 2023. https://www.aladdinsci.com/us_en/faqs/brilliant-green-agar-w-sulfadiazine-powder-en.html
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