Experimental effects of drugs on spontaneous activity in mice

Summary

Spontaneous activity is a physiological characteristic of normal animals. The amount of spontaneous activity often reflects the state of central excitation or inhibition. Sedative-hypnotic drugs such as diazepam (Valium) and other central inhibitory drugs can significantly reduce the spontaneous activity of mice; while central excitatory drugs such as caffeine can increase the spontaneous activity of mice. The purpose of this experiment is to observe the effects of drugs on the spontaneous activity of mice, in order to analyze whether the drug effects are excitatory or inhibitory.

Operation method

the analytic method

Principle

Spontaneous activity is a physiological characteristic of normal animals. The amount of spontaneous activity often reflects the state of central excitation or inhibition. Sedative-hypnotic drugs such as diazepam (Valium) and other central inhibitory drugs can significantly reduce the spontaneous activity of mice; while central excitatory drugs such as caffeine can increase the spontaneous activity of mice. The degree of increase or decrease in spontaneous activity in mice is directly proportional to the intensity of action of central excitatory or depressant drugs.

Materials and Instruments

white rat
Sodium benzoate caffeine solution
Bell jar Syringe Balance

Move

I. Methodology:

Two mice were weighed and placed in two bellows. After the mice were quiet, one was injected intraperitoneally with 150 mg∕Kg of 1% sodium benzoate caffeine solution, and the other was injected with 20 mg∕Kg of 0.5% Valium solution. 10 minutes after the administration of the drugs, the activities of the two mice were observed to see the differences.
II. Results:

Caveat

⒈ Mice should be selected as active as possible, the same sex.⒈ 24 h before the experiment, fasting and water.⒈ The mice should be as active as possible. 3) The experiment should be done at room temperature above 20 ℃.Singing is best done in the morning, because the activity of mice usually decreases at noon and in the afternoon.Be careful with observation such as walking, standing, sniffing, grooming, licking, biting, imbalance, tail erecting, etc.

Common Problems

I. Analyze the basic pharmacological effects of caffeine and Valium based on experimental results.

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