Indirect agglutination inhibition test
Indirect agglutination inhibition test
After mixing the sample to be tested with the known antibody for a certain period of time, and then mixing it with the latex particles sensitized by the corresponding antigen, if the sample to be tested contains the corresponding antigen, it can bind to the added antibody. When the sensitized latex particles are added, there is no corresponding antibody binding to the antigen on the surface of the latex particles, and thus no agglutination occurs, which is an inhibition of latex agglutination, so that those who don't agglutinate are positive, indicating that the sample to be tested contains the corresponding antigen; those who agglutinate are negative, suggesting that the sample to be tested does not contain the corresponding antigen.
Operation method
Indirect agglutination inhibition test
Principle
After mixing the sample to be tested with the known antibody for a certain period of time, and then mixing it with the latex particles sensitized by the corresponding antigen, if the sample to be tested contains the corresponding antigen, it can bind to the added antibody. When the sensitized latex particles are added, there is no corresponding antibody binding to the antigen on the surface of the latex particles, and thus no agglutination phenomenon occurs, which is a latex agglutination inhibition reaction, so those who do not agglutinate are positive, indicating that the samples to be tested contain the corresponding antigens; those who agglutinate are negative, suggesting that the samples to be tested are free of the corresponding antigens. This method is mainly used for the detection of soluble antigens, such as latex pregnancy test, is to be tested pregnant women's urine (containing human chorionic gonadotropin, HCG) and the known role of anti-HCG antibodies, thus inhibiting the anti-HCG antibodies and sensitized latex particles on the surface of the binding of HCG, and then latex agglutination is inhibited.
Materials and Instruments
Urine to be tested HCG-positive urine of pregnant women Move 1. Take a slide and place it on the laboratory bench, divide it into three compartments with a special pencil and indicate 1, 2 and 3. Latex pregnancy test Caveat 1. The diagnostic reagents used must be used within the validity period, and should be shaken well before use. 2. The dropper and the toothpick for stirring the reaction solution should not be shared. 3. the sample should not be too much, the slide should be placed flat, in order to prevent the two compartments of the reaction solution overflow mixing. 4. If the visual observation is not clear enough, the slide can be placed under the microscope with low magnification observation. Common Problems The presence of uniform, fine agglutinated particles in the first frame is considered negative (non-pregnant urine), and if it remains a homogeneous latex fluid that does not agglutinate, it is considered positive (pregnant urine). For more product details, please visit Aladdin Scientific website.
Adsorbed HCG polystyrene latex antigen Rabbit anti-human HCG immune serum (antibody) Saline
Slide Dropper Toothpick Special pencil Optical microscope
2. Take a drop of saline into the second compartment with a dropper, then take a drop of urine of the pregnant woman to be tested and add it into the first compartment, and take a drop of urine of a positive pregnant woman and add it into the third compartment.
3. Add one drop of rabbit anti-human HCG immune serum to each of the three compartments 1, 2 and 3, and mix the reaction solution with a toothpick, and leave it for 3Os to 1min.
4. Add one drop of HCG-sensitized latex antigen to each of the three compartments 1, 2 and 3, mix well with a toothpick, and leave for about lOmin to observe the results.
