10mM in DMSO for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 14 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Information
Flucytosine Flucytosine (5-Fluorocytosine, 5-FC, 5-Fluorocytosine, NSC 103805, Ro 2-9915) is an antifungal drug with IC50 of 0.93 μM in C. albicans.
In vitro
Flucytosine inhibits the growth of C. neoformans in Sabouraud\'s dextrose broth at concentrations ≥ 1.25 mg/L, and Flucytosine of 50 mg/L causes a ~50% reduction in colony-forming unit (cfu)\\ in the J774.16 killing assay with viability of J774.16 cells not affected measured by trypan blue exclusion. The combination of Flucytosine and IgGl monoclonal antibody to Cryptococcus neoformans capsular glucuronoxylomannan is more effective in reducing the numbers of C. neoformans colony-forming units in vitro with J774.16 murine macrophage-like cells than either agent alone. The efficacy of Flucytosine (5FC) in combination with amphotericin B (AB) and fluconazole (FCZ) is studied against 35 yeast isolates, of which the 5FC-FCZ combination is antagonistic against Candida species, but for some Candida isolates synergism is found.
In vivo
Administration of Flucytosine in combination with monoclonal antibody 2H1 to A/JCr mice infected with C. neoformans significantly reduces lung but not brain cfu, which is more effective than either agent alone. The combination of intravenous Flucytosine in 0.9% saline (NaCl) and amphotericin B (AmB) provides synergistic antifungal activity and is associated with a lower incidence of nephrotoxicity than with AmB treatment alone. Infusion of Flucytosine (5-10 mg/kg/min) dissolved in 5% glucose into the renal artery of an in situ perfused kidney for 15 minutes increases renal blood flow (RBF) in the rat, and the renal vasodilatation persists for the duration of the Flucytosine infusion.
Cell Data
cell lines：
Concentrations：Dissolved in PBS, final concentration 50 mg/L
Incubation Time：24 hours
Powder Purity:≥99%
| Isomeric SMILES | C1=NC(=O)NC(=C1F)N |
|---|---|
| WGK Germany | 2 |
| RTECS | HA6040000 |
| Molecular Weight | 129.09 |
| Beilstein | 127285 |
| Reaxy-Rn | 6323046 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=6323046&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Solubility | Solubility (25°C) In vitro DMSO: 50 mg/mL (77.58 mM); Water: 25 mg/mL (38.79 mM); Ethanol: 15 mg/mL (23.27 mM); |
|---|---|
| Melt Point(°C) | 297°C |
| 1. Li Wanqian, Whiteway Malcolm, Hang Sijin, Yu Jinhua, Lu Hui, Jiang Yuanying. (2025) Impairing the interaction between Erg11 and cytochrome P450 reductase Ncp1 enhances azoles’ antifungal activities. Nature Communications, 16 (1): (1-18). [PMID:40707518] [10.1038/s41467-025-62131-z] |
| 2. Meng Wang, Yanwen Zhou, Zhaohui Wang. (2023) Unearth the ultrapotent intrinsic fungicidal efficacy of the surface-piercing CuFeSe2-PVP nano-blade. CHEMICAL ENGINEERING JOURNAL, [PMID:] [10.1016/j.cej.2023.142603] |
| 3. Fan-Zhi Bu, Yue-Ming Yu, Lin-Lin Zhang, Cui-Wei Yan, Zhi-Yong Wu, Yan-Tuan Li. (2023) Salification-driven strategy toward the hydrophobic molecular salt of the antifungal drug 5-fluorocytosine and protocatechuic acid with triple-helix structure offers an innovative insight for conquering adverse drug reactions. CRYSTENGCOMM, 25 (13): (1951-1964). [PMID:] [10.1039/D2CE01523D] |
| 4. Hui Lu, Wanqian Li, Malcolm Whiteway, Hongkang Wang, Shuo Zhu, Zhe Ji, Yanru Feng, Lan Yan, Ting Fang, Liping Li, Tingjunhong Ni, Xiaolong Zhang, Quanzhen Lv, Zichao Ding, Lijuan Qiu, Dazhi Zhang, Yuanying Jiang. (2022) A Small Molecule Inhibitor of Erg251 Makes Fluconazole Fungicidal by Inhibiting the Synthesis of the 14α-Methylsterols. mBio, 14 (1): [PMID:36475771] [10.1128/mbio.02639-22] |
| 5. Fan-Zhi Bu, Yue-Ming Yu, Yu Yu, Cui-Wei Yan, Zhi-Yong Wu, Yan-Tuan Li. (2022) Self-assembly of antifungal agent 5-fluorocytosine and nutrient trans-p-coumaric acid furnishes a cocrystal with the potential to reduce toxicity and side effects for the drug: a case study combining theory with experiment. NEW JOURNAL OF CHEMISTRY, 47 (3): (1328-1341). [PMID:] [10.1039/D2NJ03622C] |
| 6. Fan-Zhi Bu, Yue-Ming Yu, Yu-Li Shen, Zhi-Yong Wu, Yan-Tuan Li. (2022) Cocrystallization with nutrient ferulic acid towards reducing the dissolubility behaviors of antifungal drug 5-fluorocytosine: An integrated theoretical and experimental case research. JOURNAL OF MOLECULAR STRUCTURE, [PMID:] [10.1016/j.molstruc.2022.134601] |
| 7. Fan-Zhi Bu, Yue-Ming Yu, Yu-Li Shen, Lu Liu, Cui-Wei Yan, Zhi-Yong Wu, Yan-Tuan Li. (2022) Cocrystallization-driven self-assembly with vanillic acid offers a new opportunity for surmounting fast and excessive absorption issues of antifungal drug 5-fluorocytosine: a combined theoretical and experimental research. CRYSTENGCOMM, 24 (15): (2777-2790). [PMID:] [10.1039/D2CE00114D] |
| 8. Zuoling Zhang, Min Xia, Pengfei Huang, Bin Di, Mengxiang Su. (2021) Preparation and evaluation of a bacitracin-bonded silica stationary phase for hydrophilic interaction liquid chromatography. MICROCHEMICAL JOURNAL, [PMID:] [10.1016/j.microc.2021.106661] |
| 9. Yang Cong, Cunbin Du, Meng Wang, Zhouyu Jiang, Mingliang Wang. (2021) Solubility of 5-Fluorocytosine in Different Pure and Binary Mixed Solvents: Measurement, Model Correlation, Solvent Effect, and Preferential Solvation. JOURNAL OF CHEMICAL AND ENGINEERING DATA, [PMID:] [10.1021/acs.jced.1c00206] |
| 10. Xue-Jie Wang, Jin-zong You, Feng Yu. (2015) Study on the thermal decomposition of emtricitabine. JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS, [PMID:] [10.1016/j.jaap.2015.08.011] |
| 11. Wang Xue-jie, You Jin-zong. (2015) Study on the thermal decomposition of capecitabine. JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY, 123 (3): (2485-2497). [PMID:] [10.1007/s10973-015-4857-9] |
| 12. Kai Li, Sinan Tian, Ke Sun, Qingguo Su, Yanhui Mei, Wenjie Niu. (2024) ROS-responsive polyprodrug micelles carrying suicide genes in combination with chemotherapy and gene therapy for prostate cancer treatment. RSC Advances, 14 (8): (5577-5587). [PMID:38352686] [10.1039/D4RA00352G] |
| 13. Jiantao Meng, Yuan Su, Hang Zhu, Jie Zhang, Ting Cai. (2025) Dynamic Molecular Cocrystals with Alkyl Chain Dependent Thermosalient Phase Transitions. Advanced Science, [PMID:40231677] [10.1002/advs.202502692] |
| 14. Meiling Nie, Ting Jiao, Lin Wang, Chiyi Wang, Jiahan Jin, Jialu Hu, Yang Li, Hailu Zhang. (2026) Functional group orientation-dependent conformational polymorphism in 5-fluorocytosine–L-malic acid cocrystals. JOURNAL OF MOLECULAR STRUCTURE, [PMID:] [10.1016/j.molstruc.2026.145506] |
