Recombinant ACTN4 Antibody - Primary antibody, for WB,IF,ICC various applications, from Human, generated in Rabbit, specific to ACTN4, IgG

Cat. No.: Ab088349
AVAILABLE TO ORDER
GRADE & PURITY Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5mg/mL
Application
IF/ICC, WB
Host species
Rabbit
Reactive species
Human, Mouse, Rat
Isotype
Rabbit IgG
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab088349-10μl
5
$86.90
50μl
Ab088349-50μl
2
$259.90
100μl
Ab088349-100μl
1
$419.90
1ml
Ab088349-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,399.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Recombinant, 0.5mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Recombinant antibody to ACTN4 could be used for WB, ICC, IF and so on.
Application
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Purity
Antigen affinity purification
Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Specifications

Product Name
Recombinant ACTN4 Antibody - Primary antibody, for WB,IF,ICC various applications, from Human, generated in Rabbit, specific to ACTN4, IgG
Synonyms
alpha Actinin 4 antibody | actinin 4 antibody | Actinin alpha 4 antibody | actinin4 antibody | ACTN 4 antibody | ACTN4 antibody | ACTN4_HUMAN antibody | Alpha-actinin-4 antibody | DKFZp686K23158 antibody | F actin cross linking proteinantibody | F-actin c
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.5mg/mL
Host Species
Rabbit
Host species
Rabbit
Specificity
ACTN4
Application
WB, IF, ICC
Immunogen
A synthetic peptide derived from human alpha Actinin 4 (AA 1-14).
Positive Control
WB: C6, NIH/3T3, HeLa, A549, K562, A431 lysates. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Antibody Type
Primary antibody
Clonality
Recombinant
Isotype
IgG
SDS-PAGE
150kDa
Purification Method
Antigen affinity purification
Source
Cell supernatant
Concentration
0.5mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

Recombinant ACTN4 Antibody (Ab088349) - Western Blot
All lanes: Recombinant ACTN4 Antibody (Ab088349) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 105 kDa
Observed band size: 103 kDa

Recombinant ACTN4 Antibody (Ab088349) - Western Blot
All lanes: Recombinant ACTN4 Antibody (Ab088349) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 105 kDa
Observed band size: 103 kDa

Recombinant ACTN4 Antibody (Ab088349) - IF
IF analysis of ACTN4 (green) in HeLa cells using Recombinant ACTN4 Antibody (Ab088349) and cells were counterstained with DAPI (blue).

Application
ApplicationDilution info
WB

1/500-1/1000

IF/ICC

1/50-1/200

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
ACTN4 Tbio Alpha-actinin-4 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0700746Certificate of AnalysisAug 01, 2023 Ab088349
ZJ23F0700747Certificate of AnalysisAug 01, 2023 Ab088349
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide →

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