GRADE & PURITYRUO?Research Use Only — for laboratory research, not diagnostic or therapeutic use. Use in R&D; do not use in clinical diagnosis or patient treatment.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Suitable for Flow Cytometry?Flow-cytometry grade — validated antibodies/reagents for FACS analysis. Use to stain and quantify cell populations by flow cytometry.0.5 mg/mL
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide com
Host species
Rat
Specificity
Mouse CD8α
Immunogen
Mouse Spleen Cells or Thymocyte Membranes
Positive Control
Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation
Pacific Blue
Excitation (nm)
Violet Laser(405nm)
Emission(nm)
455nm
Ex/Em(nm)
Ex:410nm;Em:455nm
Grade
Azide Free, ExactAb™, Recombinant, RUO, Suitable for Flow Cytometry
Action Type
ANTIBODY
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD8α expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD8α Antibody (Pacific Blue) (Ab302180) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (Pacific Blue) (Ab216768).
Flow cytometry analysis of CD8α expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD8α Antibody (Pacific Blue) (Ab302180) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (Pacific Blue) (Ab216768). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤1.0 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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