Recombinant Human Cyclin D1 Protein, >95% (SDS-PAGE)

Cat. No.: rp156643

GRADE & PURITY Carrier Free, Azide Free, His Tag, ≥95%(SDS-PAGE)

Expression System

E. coli

Accession #

P24385

Protein Tag

N-His

Expression system

E. coli

Overview

Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description：
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162, PubMed:33854235).
Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162).
Hypophosphorylates RB1 in early G1 phase (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162).
Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed:1833066, PubMed:1827756, PubMed:8302605, PubMed:19412162).
Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity (PubMed:15241418).
Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed:9106657).
Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (PubMed:16569215, PubMed:18417529).

Specifications

Product Name

Recombinant Human Cyclin D1 Protein, >95% (SDS-PAGE)

Synonyms

AI327039 | B cell CLL/lymphoma 1 | B cell leukemia 1 | B cell lymphoma 1 protein | B-cell lymphoma 1 protein | BCL 1 | BCL-1 | BCL-1 oncogene | BCL1 | BCL1 oncogene | ccnd1 | CCND1/FSTL3 fusion gene | CCND1/FSTL3 fusion gene, included | CCND1/IGHG1 fusion

Grade

Azide Free, Carrier Free, His Tag

Specifications & Purity

Carrier Free, Azide Free, His-Tag, ≥95%(SDS-PAGE)

Purity

>95% (SDS-PAGE)

Expression System

E. coli

Amino Acids

1-295 aa

Sequence

MGHHHHHHMEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI

Protein Tag

N-His

Accession #

P24385

Predicted molecular weight

34.7 kDa

SDS-PAGE

33.5 kDa, under reducing conditions

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

SDS-PAGE: Recombinant Human Cyclin D1 Protein (rp156643)

Recombinant Human Cyclin D1 Protein (rp156643) - SDS-PAGE
3 μg/lane of Recombinant Human Cyclin D1 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 33.5 kDa under reducing conditions.