GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.SUMO tag?SUMO tag grade — recombinant protein bearing a SUMO tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein. SUMO can boost expression and solubility.≥95%(SDS-PAGE)See COA
Accession #
P14174
Protein Tag
N-His & SUMO
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Immobilized Recombinant Human MIF Protein (rp170404) at 1.0 μg/mL can bind Imalumab (anti-MIF) (Ab177902) with the EC50 of 110.0 ng/mL.
Carrier Free,Bioactive,ActiBioPure™,His-Tag,SUMO tag,≥95%(SDS-PAGE),See COA ActiBioPure™,Bioactive,Carrier Free,His Tag,SUMO tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
ActiBioPure™, Bioactive, Carrier Free, His Tag, SUMO tag
Specifications & Purity
Carrier Free, Bioactive, ActiBioPure™, His-Tag, SUMO tag, ≥95%(SDS-PAGE), See COA
Biochemical and Physiological Mechanisms
MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era. Regardless, it is one of the least understood of all inflammatory mediators. Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosy
Bioactivity
Immobilized Recombinant Human MIF Protein (rp170404) at 1.0 μg/mL can bind Imalumab (anti-MIF) (Ab177902) with the EC50 of 110.0 ng/mL.
28.7 kDa, under reducing conditions; 28.7 kDa, under non-reducing conditions.
Molecule Type
Protein
Storage and Shipping
Concentration
See COA
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
Images
Recombinant Human MIF Protein (rp170404) - ELISA Immobilized Recombinant Human MIF Protein (rp170404) at 1.0 μg/mL can bind Imalumab (anti-MIF) (Ab177902) with the EC₅₀ of 110.0 ng/mL.
Recombinant Human MIF Protein (rp170404) - SDS-PAGE 3 μg/lane of Recombinant Human MIF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 28.7 kDa.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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