Recombinant Sarcosine Oxidase (SOX), CAS No.9029-22-5

CAS: 9029-22-5 Cat. No.: S774051 EC Number: 232-850-5

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥25U/mg enzyme powder; ≥40U/mg protein

Accession #

P40859

Bioactivity

≥25U/mg enzyme powder; ≥40U/mg protein

Overview

Sarcosine oxidase can catalyze the oxidative demethylation of sarcosine to generate glycine, H₂O₂, 5,10-CH2-tetrahydrofolate, which are often used in biochemical reactions.

Reaction

Product description

Appearance: Yellowish amorphous powder

Source: Microorganism

Enzyme Commission Number: EC 1.5.3.1

CAS Number: 9029-22-5

Storage temperature: -20℃

Activity: ≥25U/mg enzyme powder; ≥40U/mg protein

Unit definition: One unit will oxidase one micromole of Sarcosine per min at pH 8.0 at 37℃.

PROPERTIES
Stability:	Stable at -20℃ for at least four years
Molecular weight:	44kDa (SDS-PAGE)
Isoelectric point:	5.8
Michaelis constant:	4.3×10⁻³M (Sarcosine)
Optimum pH:	7.5-8.0	{Fig. 1}
Optimum temperature:	50℃	{Fig. 3}
pH Stability:	6.0-10.5 (25℃, 24hr)	{Fig. 2}
Thermal stability:	< 50℃ (pH 8.0, 30min)	{Fig. 4}
Inhibitors:	Cu²⁺, NEM, Proclin
Effect of various chemicals:		{Table 1}

Table 1. Effect of Various Chemicals on SOX

 [The enzyme dissolved in 50mM Tris-HCl buffer, pH 8.0 (10U/ml) was incubated with each chemical at 37℃ for 2hr.]

  

Specifications

Product Name

Recombinant Sarcosine Oxidase (SOX), CAS No.9029-22-5

Synonyms

Sarcosine: oxygen oxidoreductase (demethylating) | sarcosine oxidasee (formaldehyde-forming) | monomeric sarcosine oxidase | MSOX | sarcosine oxidasee | soxA

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥25U/mg enzyme powder; ≥40U/mg protein

Biochemical and Physiological Mechanisms

Catalyzes the oxidative demethylation of sarcosine. Can also oxidize other secondary amino acids such as N-methyl-L-alanine.

Bioactivity

≥25U/mg enzyme powder; ≥40U/mg protein

Accession #

P40859

Predicted molecular weight

44 kDa

CAS

9029-22-5

Enzyme Commission Number

EC 1.5.3.1

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥25U/mg enzyme powder; ≥40U/mg protein

Reconstitution

Reconstitute in water.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (4 years). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Unit definition

One unit will oxidase one micromole of Sarcosine per min at pH 8.0 at 37℃.

References

1. Ziyi Tong, Shengyan Hou, Zhenkun Zhang, Zhen Liu, Yifei Zhang.  (2024)  Biochemical approaches for decoding the information stored with metabolites.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.136618]

2. Yan Tao, Chongying Liao, Fangli He, Yixuan He, Sihui Yang, Liu-Pan Yang, Li-Li Wang.  (2025)  Smartphone-assisted colorimetric sensing platform based on Fe-LCDs nanozymes for detection of creatine in meat.  FOOD CHEMISTRY,      [PMID:40381557] [10.1016/j.foodchem.2025.144754]

3. Fangbing Wang, Qiwen Peng, Yongheng Zhang, Min Zhang, Guoyue Shi.  (2026)  Five-in-one colorimetric multiplexed point-of-care testing of blood biomarkers via Janus separation-sensing membrane.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2026.174104]