EnzymoPure™, 50U/μl EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Thermostable T7 RNA polymerase is a genetically engineered T7 RNA polymerase. The enzyme highly specifically recognizes the T7 promoter sequence and can be highly transcribed in vitro at 50 ℃. In the process of transcription in vitro, it can improve the capping efficiency and eliminate the production of dsRNA by-products.
Product features
It has high specificity for T7 promoter and is used for the synthesis of RNA (including microRNA) in vitro.
Product usage
1) Synthesis of single stranded RNA;
2) Synthesis of highly specific RNA probes;
3) Synthetic siRNA precursor;
4) Making the precursor of RNA splicing reaction;
5) Cap analog was used as primer to make capped mRNA.
Usage suggestions
For efficient transcription of a specific region, it is suggested that the template DNA be pre cut into flat ends or 5 'protruding ends downstream of the region.
Spermidine in the buffer may combine with nucleic acid to form insoluble substance. It is suggested to add template DNA finally.
Storage temperature
-Long term storage at 20 ℃ or -80 ℃.
Quality assurance
After column purification for many times, only a clear and single target band can be seen by SDS-PAGE gel detection, with a purity of 95%. No E. coli DNA residue, RNase, endonuclease and exonuclease contamination can be detected by PCR.
Activity definition
Under the condition of 50 ℃ and ph8.0, the amount of enzyme required to add 1nmol of [3h] GMP into the acid insoluble precipitate within 1 hour is defined as 1 active unit.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 B2327171 | Certificate of Analysis | Mar 18, 2026 | T489384 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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