Articles by Topic "PCR"

1. Types, Properties, and Applications of Commonly Used Polymerases in Molecular Biology Experiments
   posted this at May 20, 2026

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   Polymerases are core enzymes used in nucleic acid replication, amplification, sequencing, labeling, reverse transcription, and in vitro transcription. Different polymerases vary significantly in template compatibility, thermostability, extension rate, fidelity, strand-displacement activity, ...
2. DNA Polymerases: Structural and Functional Features, Key Types, and Application Guidance
   posted this at Jan 21, 2026

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   DNA polymerases are the central enzymes that catalyze the extension of deoxyribonucleic acid (DNA) chains, supporting core biological processes such as DNA replication, repair, and recombination. In vitro molecular biology and biopharmaceutical analytical workflows, DNA polymerases are the ...
3. A Review of Ten Commonly Used Techniques for Detection, Quantification, and Interaction Analysis in Nucleic Acid Research
   posted this at Jan 21, 2026

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   Nucleic acid research spans confirmation of DNA/RNA presence, sequence-level characterization, and mechanistic dissection of interactions between nucleic acids and proteins or other nucleic acids. The choice of experimental method determines evidence type, resolution, throughput, and ...
4. Principles and Application Strategies of Common PCR Enhancers
   posted this at Dec 22, 2025

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   PCR is a foundational technique in molecular biology and molecular diagnostics. However, high-GC/high-AT templates, long-amplicon amplification, complex sample matrices, and residual inhibitory substances from extraction often reduce amplification efficiency or lead to complete failure. PCR ...
5. Introduction to Various Types of PCR Technology
   posted this at Jun 9, 2025

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   Polymerase Chain Reaction (PCR) is an experimental technique capable of rapidly and efficiently amplifying specific DNA fragments. It consists of three fundamental steps: high-temperature denaturation, low-temperature annealing, and optimal-temperature extension. By cycling through these steps ...
6. PCR Laboratory Contamination Prevention and Efficient Reagent Application: —Optimizing Experimental Steps and Choosing
   posted this at Jun 9, 2025

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   Polymerase Chain Reaction (PCR) is a core technology in molecular biology, clinical diagnostics, and scientific research. Its high sensitivity means that even minute contaminants can affect experimental results, leading to false positives or false negatives.
7. PCR, MIQE Guidelines
   posted this at Jun 9, 2025

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   The MIQE Guidelines:Minimum Information for Publication of Quantitative Real-Time PCR Experiments