GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥95%(SDS-PAGE)Protein concentration: See COA; ≥10 U/mg protein; expressed in E.coli
Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥95%(SDS-PAGE),Protein concentration: See COA; ≥10 U/mg protein; expressed in E.coli ActiBioPure™,Bioactive,High Performance,Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
UDP-sugar pyrophosphorylase (BlUSP, E.C. 2.7.7.64) is a key enzyme involved in the biosynthesis of sugar nucleotides in organisms. Derived from Bifidobacterium longum, it catalyzes the formation of UDP-sugars (e.g., UDP-glucose, UDP-galactose), which serve as glycosyl donors for the biosynthesis of various biomacromolecules.In combination with inorganic pyrophosphatase and Galactokinase (Cat. No. G1434912), this enzyme is used to synthesize UDP-galactose and its derivatives as glycosyl donors. This product is recombinantly expressed in Escherichia coli, featuring high purity and good compatibility, suitable for one-pot multi-enzyme systems.
Reaction:
Protocol for Use
Add substrates to the reaction mixture, supplemented with 5 mM MgCl₂. An appropriate buffer (e.g., Tris-HCl) may be added to adjust the pH to 7.5–8.5, followed by the addition of an appropriate amount of BlUSP (adjust according to reaction requirements).
Mix thoroughly and incubate the sample at 37°C for 30–60 minutes. The enzyme exhibits maximum activity in near-neutral pH buffers. The recommended reaction buffer is Tris-HCl (pH 8.0); other common biological buffers such as Tris or PBS are also applicable.
Note: For scaled-up reactions, adjust the incubation time or enzyme amount based on actual conditions. Gentle shaking may be used for mixing. Addition of inorganic pyrophosphatase can improve the conversion rate.
Precautions
For your safety and health, please wear lab coats and disposable gloves when handling. This product is for research use only!
Specifications
Product Name
UDP-sugar pyrophosphorylase (BlUSP), CAS No.223918-15-8
ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™
Specifications & Purity
Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥95%(SDS-PAGE), Protein concentration: See COA; ≥10 U/mg protein; expressed in E.coli
Bioactivity
≥10 U/mg protein
Predicted molecular weight
67.9 kDa
CAS
223918-15-8
Enzyme Commission Number
EC 2.7.7.64
Molecule Type
Enzyme
Storage and Shipping
Concentration
Protein concentration: See COA; ≥10 U/mg protein; expressed in E.coli
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Unit definition
One unit is defined as the amount of enzyme that catalyzes the production of 1 μmol of UDP-Gal from Gal-1-P and UTP per minute at 37°C.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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