UltraBio™ Small DNA Clean Beads - BioReagent, DNase, RNase free, Suitable for molecular biology, for DNA and RNA applications

Cat. No.: S1456145

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, for DNA and RNA applications

Synonyms

Smarter DNA Clean beads

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Application

Nucleic Acid Extraction and Purification

Overview

UltraBio™ Small DNA Clean Beads designed for efficient purification of DNA fragments ≥50 bp. Enables rapid removal of primer dimers, dNTPs, salts, and proteins. Purified DNA is directly suitable for sequencing, PCR templates, enzymatic digestion, and other downstream applications.

User-Supplied Reagents & Equipment

1.Reagents

80% Ethanol

Elution Buffer: 10 mM Tris-HCl (pH 8.0) or Nuclease-Free Water

2.Equipment

Pipettes

Vortex mixer

Magnetic rack

1.5 mL microcentrifuge tubes

Manual Protocol (Centrifuge Tubes)

                       Table 1: Bead-to-Sample Ratios

DNA Fragment Size	≥50 bp	≥200 bp	≥500 bp
Bead Suspension Volume	2.2×	1.0×	0.5×

1.Binding

Add bead suspension (volume per Table 1) to sample in 1.5 mL tube.

Vortex-mix → Incubate 5 min at RT.

Place tube on magnetic rack → Discard supernatant when clear.

2.Wash 1

Keep tube on rack. Add 200 μL 80% ethanol (submerge beads).

Incubate 1 min → Discard supernatant (do not resuspend).

3.Wash 2

Repeat Step 2.

4.Ethanol Removal

Air-dry beads 2–5 min (critical: avoid over-drying).

5.Elution

Add 30–100 μL Elution Buffer.

Vortex-mix → Incubate 5 min at RT.

6.DNA Recovery

Place tube on magnetic rack 2–5 min.

Transfer supernatant to new tube.

Automated Protocol: 16/32-Channel System

                                                                       96-Well Plate Setup

Well Position	1、7	2、8	3、9	6、12
Reagent	Sample + Beads (vol. per Table 1)	200 μL 80% ethanol	200 μL 80% ethanol	30–100 μL Elution Buffer

Procedure

1.Load plate into instrument → Insert magnetic tips.

2.Execute predefined program.

3.Transfer eluate from columns 6 & 12 to nuclease-free tubes.

Automated Protocol: 96-Channel System

                                       96-Well Plate Setup

Plate Position	1	2	3	6
Reagent per Well	Sample + Beads (vol. per Table 1)	200 μL 80% ethanol	200 μL 80% ethanol	40–100 μL Elution Buffer

Procedure

1.Load plate → Insert magnetic tips.

2.Run instrument program.

3.Seal elution plate or transfer to nuclease-free PCR plate.

Storage: -20°C

Precautions

1.Bead Handling

DO NOT freeze or centrifuge the suspension.

Vortex thoroughly before first use.

2.Sample Volume

Minimum 100 μL (adjust to 100 μL with water if needed).

3.Pre-Use Protocol

Equilibrate beads to RT for ≥30 min.

4.Critical Step

Avoid over-drying beads during ethanol removal.

Specifications

Synonyms

Smarter DNA Clean beads

Specifications & Purity

BioReagent, DNase, RNase free, Suitable for molecular biology, for DNA and RNA applications

Stability And Storage

Store at 2-8℃ long term (12 months). Do not freeze.

Storage

Store at 2-8°C, Do not freeze

Shipped In

Wet ice, Do not freeze

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, DNase, RNase free, for DNA and RNA applications, Suitable for molecular biology