5(6)-Tetramethylrhodamine isothiocyanate - for fluorescence analysis, Ex:552nm, Em:578nm, mixture of isomers , CAS No.95197-95-8

CAS: 95197-95-8 Cat. No.: T131567 Molecular Weight: 443.52 EC Number: 635-450-2 PubChem CID: 163138301
AVAILABLE TO ORDER
GRADE & PURITY for Fluorescence analysis ? Fluorescence-analysis grade — very low fluorescent impurities for clean spectra. Use in fluorescence assays where background interferes. Ex:552nm ? Fluorescence excitation maximum at 552 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:578nm ? Fluorescence emission maximum at 578 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. mixture of isomers
Synonyms
TRITC | MRITC | 5(6)-TRITC | Tetramethylrhodamine-5(6)-isothiocyanate | Tetramethylrhodamine B isothiocyanate | Tetramethylrhodamine isothiocyanate mixed isomers
Storage
Store at -20°C,Argon charged
Shipped In
Ice chest + Ice pads
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
5mg
T131567-5mg
3

$235.90

$274.90
Save $39.00 (14.19%)
25mg
T131567-25mg
3

$926.90

$1,091.90
Save $165.00 (15.11%)
100mg
T131567-100mg
2

$2,942.90

$3,433.90
Save $491.00 (14.30%)
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Why this grade

for fluorescence analysis, Ex:552nm, Em:578nm, mixture of isomers Em:578nm,Ex:552nm,for Fluorescence analysis for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Argon charged Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 6 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Tetramethylrhodamine isothiocyanate mixed isomers is an amino-reactive labeling reagent. 5(6)-TRITC is widely used in preparing bioconjugates of proteins and nucleic acids.
An amino-reactive labeling reagent.


application:

5(6)-TRITC is widely used in preparing bioconjugates of proteins and nucleic acids. It is suitable for protein, peptide and nucleotide labeling. Serves as an acceptor for FRET. It is recommended as a donor molecule in these FRET-based applications.

Specifications

Synonyms
TRITC | MRITC | 5(6)-TRITC | Tetramethylrhodamine-5(6)-isothiocyanate | Tetramethylrhodamine B isothiocyanate | Tetramethylrhodamine isothiocyanate mixed isomers
Specifications & Purity
for fluorescence analysis, Ex:552nm, Em:578nm, mixture of isomers
Biochemical and Physiological Mechanisms
Amino-reactive bright orange-red fluorophore. Suitable for protein, peptide and nucleotide labeling. Serves as an acceptor for FRET. Mixed 6-TRITC and 5-TRITC isomers. Shows spectral similarities to 5(6)-TAMRA.
Storage
Store at -20°C, Argon charged
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
Em:578nm, Ex:552nm, for Fluorescence analysis
Note
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Names and Identifiers
WGK Germany 3
PubChem CID 163138301
Molecular Weight 443.52

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

8 results found

Lot NumberCertificate TypeDateItem
F2605130Certificate of AnalysisJun 09, 2026 T131567
A2307050Certificate of AnalysisMay 20, 2026 T131567
A2307066Certificate of AnalysisMay 20, 2026 T131567
A2307067Certificate of AnalysisMay 20, 2026 T131567
G2524095Certificate of AnalysisMay 20, 2026 T131567
A2115109Certificate of AnalysisNov 10, 2022 T131567
B2224621Certificate of AnalysisFeb 10, 2022 T131567
B2224639Certificate of AnalysisFeb 10, 2022 T131567
Chemical and Physical Properties
SolubilitySoluble in DMSO or methanol
SensitivityLight, Air & Moisture Sensitive
Documents & Articles
Citations of This Product
References
1. Hong Li, Xiaodan Shi, Wei Cui.  (2022)  Self-assembled asparaginase-based nanoparticles with enhanced anti-cancer efficacy and anticoagulant activity.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,      [PMID:] [10.1016/j.colsurfa.2022.128933]
2. Xiaoqing Liu, Yiming Sun, Jie Wang, Yongyuan Kang, Zhaolong Wang, Wangbei Cao, Juan Ye, Changyou Gao.  (2024)  A tough, antibacterial and antioxidant hydrogel dressing accelerates wound healing and suppresses hypertrophic scar formation in infected wounds.  Bioactive Materials,      [PMID:38261887] [10.1016/j.bioactmat.2023.12.019]
3. Xiudan Wang, Rui Tian, Chenghong Liang, Yifan Jia, Lingyun Zhao, Qinyuan Xie, Fenglian Huang, Huijuan Yuan.  (2024)  Biomimetic nanoplatform with microbiome modulation and antioxidant functions ameliorating insulin resistance and pancreatic β-cell dysfunction for T2DM management.  BIOMATERIALS,      [PMID:39236631] [10.1016/j.biomaterials.2024.122804]
4. Ke Yang, Li Wang, Jinyi Chen, Zefeng Wang, Junqin Li, Xi Chen, Shuting Fu, Luo Hai, Le Deng, Dinggeng He.  (2024)  H2O2-Activatable Liposomal Nanobomb Capable of Generating Hypoxia-Irrelevant Alkyl Radicals by Photo-Triggered Cascade Reaction for High-Performance Elimination of Biofilm Bacteria.  Advanced Healthcare Materials,      [PMID:39155413] [10.1002/adhm.202402136]
5. Han Zu, Fang Pan, Heng-Li Fan, Zhi-Gang Qian, Xiao-Xia Xia.  (2025)  Dynamic Structural Organization of Biomolecular Condensates in Escherichia coli Cells.  JACS Au,      [PMID:41169543] [10.1021/jacsau.5c00600]
6. Hao Liguo, Yin Qiangqiang, Song Jianing, Yu Xiaoyang, He Jialong, Kang Yu-Ming.  (2026)  The upregulated RNA circ_0060055 regulates the proliferation, invasion and apoptosis of pancreatic cancer cells through spongy miR-1298-5p.  MEDICAL ONCOLOGY,  43  (3): (127).  [PMID:41615581] [10.1007/s12032-026-03278-7]
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Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View for Fluorescence analysis grade guide → View Ex:552nm grade guide → View Em:578nm grade guide →

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