Animal model of cartilage defects in the knee joint

Summary

Since the knee joint is the largest joint in the body, its range of motion is large, the soft tissue coverage is extensive, and the synovial tissue is also the most abundant, which makes it the most likely to have cartilage defects in the clinic. Moreover, the knee joint has less soft tissue, which is not only conducive to the replication of the model, but also easy to be exposed and observed, so it is often chosen as an animal model of cartilage defects in the knee joint, which is of great practicality and scientificity.

Principle

The basic principle of the [Animal model of knee cartilage defect] is to select the appropriate size of cartilage on the cartilage surface of the knee joint according to the needs of the experiment, and then directly excise the cartilage under direct vision or arthroscopy to cause defects in the cartilage tissue of the knee joint.

Operation method

Animal model of cartilage defects in the knee joint

Principle

The basic principle of [Animal model of knee cartilage defect] is to select the appropriate size of cartilage on the cartilage surface of the knee joint according to the needs of the experiment, and then directly excise the cartilage under direct vision or arthroscopy to cause defects in the cartilage tissue of the knee joint.

Materials and Instruments

Material: Adult healthy rabbit
Reagents:
① Sodium pentobarbital or uradantin
② Toluidine blue

Move

The modeling process of the knee cartilage defect animal model can be divided into the following steps:
A. Adult healthy rabbits were selected. Anesthesia was administered intravenously or intraperitoneally with sodium pentobarbital or urachal.
B. Under sterile conditions, a curved incision was made on the medial side of the knee, and the patella was pushed to the lateral side to reveal the articular surface of the femoral condyles, and a full-length cartilage defect (6 mm × 8 mm in size) was created with a sharp knife on the opposite side of the patella to a depth of 6 mm × 8 mm, with the depth of uniform and active bleeding throughout the entire trauma.
C. The resected cartilage was stained with toluidine blue, and observed to see whether it was a full-length defect or not.

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