Animal model of infected bone defects

Summary

Bone infections can lead to osteomyelitis, a bone disease that is second only to cancer in terms of difficulty in management and high recurrence rate. Osteomyelitis develops when there is a chronic bone infection over a long period of time. Osteomyelitis is usually caused by trauma and is difficult to cure clinically. Its pathology centers on the formation of dead bone, and its course is usually prolonged and persistent, and it is common in clinical practice. The main causative agent is Staphylococcus aureus.

Principle

The basic principle of [Infectious Bone Defect Animal Model] is to use the standard strain of Staphylococcus aureus, which is commonly found in infectious bone defects, to prepare a model of infectious bone defects, and then review the X-ray regularly, the formation of infected defects, and observe the formation of sinus tracts and secretions, which can also be used to study the mechanism of bacterial biofilm in vivo for resisting infections.

Operation method

Animal model of infected bone defects

Principle

The basic principle of [Infectious Bone Defect Animal Model] is to use the standard strain of Staphylococcus aureus, which is commonly found in infected bone defects, to prepare a model of infected bone defects, and then review the X-ray regularly, the formation of infected defects, and observe the formation of sinus tracts and secretions, which can also be used to study the mechanism of bacterial biofilm in vivo for resisting infections.

Materials and Instruments

Equipment: Adult healthy rabbit, 1.5 mm Crixon needle
Reagents:
① 3% pentobarbital injection
② Povidone-iodine
① 3% pentobarbital injection ② povidone iodine ③ 5% sodium cod liver oil
④ Staphylococcus aureus solution (2 × 10)
6 ④ Staphylococcus aureus solution (2×10-6 CFU)
Staphylococcus aureus solution (2×10 6 CFU)
⑤ Physiological saline
⑥ Sterile bone wax
⑦ ATCC Staphylococcus aureus standard strain

Move

The basic procedure for modeling infected bone defects can be divided into the following steps:
A. Select healthy adult rabbits and anesthetize them with 3% pentobarbital injection 1 m/kg intravenously from the ear margins.
B. The rabbit was fixed in the supine position with limbs adducted, head tilted back, sterilized by iodine, and sterile slips were placed on the rabbit.
C

. Purchase enlarged cultures, and make a 1 cm incision below the knee on the lateral side of the hind limb under anesthesia, exposing the tibia and punching holes into the proximal end of the tibia with a 1.5-mm Gram's needle. The tibia is exposed and the proximal end of the tibia is perforated with a 1.5 mm grinder.

D. Inject an appropriate amount of 5% sodium cod liverate into the medullary cavity.

E

. After 1 minute, inject 5 ul of Staphylococcus aureus solution ( ^{2×106 CFU} ) into the medullary cavity.

F. The syringe was rinsed with 0.1 ml of saline and injected into the medullary cavity to ensure that all S. aureus entered into the medullary cavity.

G. The hole was closed with sterile bone wax, and the contralateral hind limb was sutured to the skin in the same way.
H. All the rabbits were observed for 4 weeks after the operation. All rabbits were implanted with ATCC standard strain of S. aureus.

For more product details, please visit Aladdin Scientific website.

https://www.aladdinsci.com/