Uric acid is the product of purine metabolism. Hyperuricemia and uric acid nephropathy are common diseases in middle-aged and elderly men in the world today, which are caused by abnormal purine metabolism. There is no ideal animal model for hyperuricemia and uric acid nephropathy. At present, there are 1 kinds of animal molels of hyperuricemia and hyperuricemic nephropathy modeling methods: animal molels of hyperuricemia and hyperuricemic nephropathy.
Principle
Depending on the experimental method, the corresponding principles are different:
The basic principle of animal model of hyperuricemia and uric acid nephropathy:
In this experiment, rats were fed with different doses of adenine to detect changes in blood uric acid, uric acid and renal function, and to observe renal histopathology, morphology and ultrastructure, the results showed that the animal model of hyperuricemia and uric acid nephropathy was successful.
Appliance
The common application areas of animal models of hyperuricemia and uric acid nephropathy are as follows: the rat model of hyperuricemia and uric acid nephropathy established with medium-dose histadenine can be used to study the mechanism of nephropathy caused by hyperuric acid and observe the preventive and therapeutic effects of drugs.
Operation method
Animal models of hyperuricemia and uric acid nephropathy
Principle
In this experiment, rats were fed with different doses of adenine (adenine), respectively, and blood uric acid, uric acid and renal function changes were detected, and the observation of renal histopathological morphology and ultrastructure was carried out, and the results showed that the animal model of hyperuricemia and uric acid nephropathy was successful.
Materials and Instruments
Subjects: Move The basic process of the animal model of hyperuricemia and uric acid nephropathy can be divided into the following steps: Caveat 1. The blood uric acid level of the middle-dose group and the high-dose group increased significantly on the 7th day; the blood uric acid level of the low-dose group did not increase significantly; with the prolongation of time, the blood uric acid level of the middle-dose group and the high-dose group decreased gradually; the blood uric acid level of the treatment group did not increase on the 3rd day and the 7th day; the concentration of uric acid of the middle-dose group and the high-dose group increased significantly on the 3rd day; the concentration of uric acid of the middle-dose group and the high-dose group started to decrease from the 14th day onwards, but the decrease was not significant. In the low-dose group, the uric acid concentration started to increase on the 3rd day; in the allopurinol group, there was no significant change in the uric acid concentration. BUN and Cr increased significantly in the middle-dose group and high-dose group on the 10th day, but the changes were not obvious in the low-dose group and the treatment group; the amount of serum protein decreased significantly in the treatment group on the 10th day after the experiment; the results of protein measurement of the rest of the groups did not have any obvious changes at different times.Pathological examination showed that on the 14th day after the experiment, inflammatory cell infiltration in the renal interstitium, tubular dilatation, interstitial nephritis and needle crystals could be seen in the middle-dose group and the high-dose group, while there was no obvious change in the control group, the low-dose group and the treatment group. For more product details, please visit Aladdin Scientific website.
① Rats.
Experimental reagents:
① Adenine;
② Purinol;
③ 10% formaldehyde.
60 male Wistar rats, weighing 250 (±10) g, were randomly divided into the low-dose group, the medium-dose group, the high-dose group and the control group. In the medium-dose group (hereinafter referred to as the treatment group), there were 12 animals in each group, all of which were given free water, and the experiment was started after 1 week of acclimatization. The control group was fed with regular feed, and the low-dose group, medium-dose group and high-dose group were fed with adenine 1 g/kg, 2 g/kg and 4 g/kg, respectively, with 10 g of adenine per day per rat, and the shortfall was fed with regular feed; in the treatment group, in addition to 10 g of adenine 2 g/kg per day per rat, allopurinol was given by gavage, and the animals were treated with adenine 2 g/kg per day per rat on the 3rd day before the experiment and on the 3rd day after the experiment. Blood and urine were collected on the 3rd, 7th, 10th, 14th and 21st days before the experiment. On the 14th day of the experiment, 4 rats in each group were randomly selected to be killed alive and the kidney tissues were fixed in 10% formaldehyde and sent for pathological examination.