Animal models of vitamin A deficiency

Summary

Vitamin A deficiency is a systemic disease caused by the lack of vitamin A in the body, mainly eye and skin lesions, most common in children aged 1-4 years; the earliest symptom is poor dark adaptation, dry eye conjunctiva and cornea, and later develops into corneal softness and dry skin and follicular keratinization, so it is also known as night blindness, dry eye, corneal softening disease. Vitamin A deficiency is also teratogenic to the heart, and maternal vitamin A deficiency can lead to a variety of cardiovascular malformations in the embryo. For the study of vitamin A deficiency, various animal models of vitamin A deficiency have been replicated, such as rat, mouse, and free. Among them, the rat is the most common. This model is characterized by short formation period, stable model, feasibility and reproducibility.

Principle

Vitamin A deficiency is induced in the organism by removing vitamin A from the animal's diet and referral water.

Appliance

It is mainly used for research on the effects of vitamin A deficiency on organ development and the effects of vitamin A deficiency during pregnancy on the fetus.

Operation method

Establishment of an animal model of vitamin A deficiency

Principle

Vitamin A deficiency is induced in the organism by removing vitamin A from the animal's diet and referral water.

Materials and Instruments

Animal models:
Newly weaned SD-grade or Wistar clean rats;
Equipment:
One set of conventional surgical instruments.
Reagents:
Anesthetic (chloral hydrate).

Move

Vitamin A deficiency animal model was established in the following steps:

(i) 21-day-old weanling SD-grade or Wistar-cleaned rats: A

. Laboratory acclimatization feeding for 1-2 weeks, the feed was based on the purified feed formula for growing rodents (AIN-93 G), the vitamin A content was removed, and the animals were free-feeding and drinking distilled water

.

B. During the 8-week experimental period, serum vitamin A was determined by high-performance liquid chromatography (HPLC).

(B) Modeling of pregnant rats and lactating mice: A

. Female rats were fed with vitamin A-deficient diets for 3 weeks and mated with male rats of normal feeding conditions, and then checked for negative plugs early in the morning of the 2nd day.

B. (2) Cervical vertebrae were dissected from 20-day-old females, and liver and heart tissues from embryonic rats were dissected, and lung tissues from 1-day-old mammary rats (average 22 days of gestation) were dissected.

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