Experiments on the observation of the inhibitory and excitatory effects of drugs on respiration
Experiments on the observation of the inhibitory and excitatory effects of drugs on respiration
High-dose injections of aminoglycoside antibiotics can produce a blockade of the muscle junction, which is manifested by weakness of the extremities. Dyspnea or even respiratory arrest. Calcium or neostigmine may antagonize this toxic effect. Pentobarbital sodium blood concentration increased to a certain level can inhibit the respiratory center, early respiratory depression can be antagonized by nikshamil.
Operation method
antagonistic method
Principle
High-dose injections of aminoglycoside antibiotics can produce a blockade of the muscle junction, which is manifested by weakness of the extremities. Dyspnea or even respiratory arrest. Calcium or neostigmine may antagonize this toxic effect. Pentobarbital sodium blood concentration increased to a certain level can inhibit the respiratory center, early respiratory depression can be antagonized by nikshamil.
Materials and Instruments
Rabbits Move 1. A rabbit was taken, weighed and anesthetized, and fixed in the supine position on a rabbit table. For more product details, please visit Aladdin Scientific website.
Gentamicin solution Calcium gluconate solution; sodium pentobarbital; niclosamide
Rabbit table Rabbit fixation cords Skin scissors Wire scissors Hemostatic forceps Tissue forceps Ophthalmic scissors Silk thread Ophthalmic forceps Syringes Needles
2. Cut a 3-5 cm longitudinal incision along the abdominal white line centered on the raphe, expose the cartilage under the raphe, gently lift the raphe with hemostatic forceps, carefully turn it over, expose the diaphragm muscle bundle attached under the raphe, carefully separate the diaphragm muscle bundle with curved ophthalmologic forceps, thread it, and ligature the diaphragm muscle bundle, and, finally, dissociate the raphe from the sternum and leave it free.
3. Connect the other end of the wire with the tension transducer, adjust the tension of the wire so that it can clearly reflect the amplitude and frequency of respiratory movements in the computer, and record a normal respiratory curve.
4. 4% gentamicin (1 ml/Kg) was injected intravenously, and the respiratory changes of the rabbits were observed. When the rabbits' respiration was obviously suppressed, 2.5% niclosamide was given at 2-3 ml/kg, and whether the respiration was restored was observed, then 10% calcium gluconate (1 ml/Kg) was given, and the respiratory changes of the rabbits were again observed, and the differences between the two were compared.
5. After the respiration recovered to normal, 3% pentobarbital sodium 0.5 ml/kg was injected very slowly and the respiratory rate was observed. When respiratory depression was obvious, 2.5% niclosamide 2-3 ml/kg was injected immediately until respiration was restored.
6. The curve of respiratory changes was edited in the computer, then printed and analyzed the results.
Precautions: when separating and ligating the diaphragm, a pneumothorax should be avoided, and the action should be gentle to avoid damaging the diaphragm strips; intravenous gentamicin should be injected slowly; its toxic reaction usually begins to appear after 5 min, and gradually aggravates.