Feulgen staining of DNA

Summary

DNA is hydrolyzed by a weak acid (1 mol/L HCl), and the bonds between the purine bases on it and the deoxyribose sugar are opened, causing the formation of free aldehyde groups on one end of the deoxyribose, and these aldehyde groups react in situ with Schiff's reagent (a colorless magenta sulfite solution) to form a purplish-red compound that gives a purplish-red-positive reaction to the part of the cell containing the DNA. The violet-red color is due to the fact that the reaction product contains a quinone group within the molecule, which is a chromophore and therefore has a color. The control group was pre-treated with hot trichloroacetic acid or DNAase, and the negative reaction was obtained by extracting the DNA from the cells, thus demonstrating the specificity of the Feulgen reaction. <

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