Modeling experiments in animal models of chronic bronchitis
Modeling experiments in animal models of chronic bronchitis
Chronic bronchitis, a chronic nonspecific inflammation of the bronchial tubes, bronchial mucosa and their surrounding tissues, is a common disease that seriously jeopardizes the health of middle-aged and elderly people, with a prevalence rate of about 15% among people over 50 years old in China. Due to the complex etiology of chronic bronchitis, the variety of clinical symptoms and repeated delays, which bring great difficulties in the reasonable simulation of animal models and the objective, precise and quantitative observation indexes, the progress of research is very slow, and most of the studies are still stuck in the application of the traditional classical methods, and in recent years, although there are new explorations, the practical ones are very few. Currently, there are five main methods for modeling chronic bronchitis: exposure to smoke, SO2, lipopolysaccharide (endotoxin), enzymes, and adrenergic and cholinergic drugs or pro-secretin.
Principle
Smoking method to build the basic principle of chronic bronchitis animal model is smoking smoke caused by long-term stimulation of the respiratory tract is the most important etiology of chronic bronchitis, paper cigarettes contain tar and nicotine can increase the parasympathetic excitability, so that the bronchial contraction spasms, increase airway resistance; at the same time, the bronchial mucosal glands hyperplasia and hypertrophy, cupular cell hyperplasia and squamous epithelial cell metaplasia, increased secretion of the mucosa and the accumulation of Epithelial cell cilia movement is inhibited; mucosal congestion, edema, alveolar phagocytosis function is reduced, so that the airway purification capacity is weakened, are prone to cause infection. The longer the smoking time, the larger the amount of smoke, the higher the prevalence.
Operation method
Construction of an animal model of chronic bronchitis by fumigation method
Principle
Smoking method to build the basic principle of chronic bronchitis animal model is smoking smoke caused by long-term stimulation of the respiratory tract is the most important etiology of chronic bronchitis, paper cigarettes contain tar and nicotine can increase the parasympathetic excitability, so that the bronchial contraction spasms, increase airway resistance; at the same time, the bronchial mucosal glands hyperplasia and hypertrophy, cupular cell hyperplasia and squamous epithelial cell metaplasia, increased secretion of the mucosa and the accumulation of Epithelial cell cilia movement is inhibited; mucosal congestion, edema, alveolar phagocytosis function is reduced, so that the airway purification capacity is weakened, are prone to cause infection. The longer the smoking time, the larger the amount of smoke, the higher the prevalence.
Materials and Instruments
Equipment: Move The basic process of constructing an animal model of chronic bronchitis by smoking can be divided into the following steps: For more product details, please visit Aladdin Scientific website.
① 50 mL syringe;
② Cigarette;
③ 1000 mL lower neck bottle;
④ Rats/mice.
(I) Rats
A. Smoking 25 paper cigarettes, 4 h/d for 2-6 weeks replicated into a subacute chronic bronchitis model, which initially induced basal cell hyperplasia, followed by plasma cell chemotaxis of the superficial epithelium and hyperplasia of newly-formed mucus cells and thickening of the epithelium due to cellular hypertrophy. There was also laryngeal and proximal airway submucosal gland hypertrophy with increased tracheal mucus volume, and proliferation of proximal and terminal airway epithelium and alveolar epithelium.
After cessation of smoking, the number of secretory cells in the trachea, proximal airways and distal fine bronchioles could be gradually restored to the control level.
(II) Mice
A. Mice were placed in a 1000 ml bottle with a 1.5 cm diameter vent hole in the cap, a three-way tube was connected to the lower port, and a 50 mL syringe and a lighted cigarette were connected to the other two ends, and the syringe was used to continuously inhale cigarette smoke through the three-way tube, and the smoke was smoked for 30 minutes at a time of 400 mL (the concentration of smoke in the bottle was about 4%).
B. The smoke was smoked once each time in the afternoon and once each time in the afternoon for the first 10 days, and once each time in the afternoon for the next 10 days. B. Smoke once in the afternoon of the first 10 days and once in the afternoon of the second 10 days, 30 times in a row for 20 days.